Controllable Assembly of Enzymes for Multiplexed Lab-on-a-Chip Bioassays with a Tunable Detection Range

Angewandte Chemie
Yunlei XianyuXingyu Jiang

Abstract

Multiplexed analysis of molecules with different concentrations requires assays with a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in a controlled fashion to generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly of enzymes on detection antibodies allows for lab-on-a-chip immunoassays with a tunable detection range from pg mL-1 to μg mL-1 . Simultaneous, multiplexed bioassays of clinically relevant inflammatory biomarkers in serum are demonstrated in one lab-on-a-chip format, with a limit of detection of 0.47 pg mL-1 for interleukin-6, 2.6 pg mL-1 for procalcitonin, and 40 ng mL-1 for C-reactive protein. This controlled assembly technique provides a multiplexed platform for simultaneous and quantitative analyses of both low-abundance and high-abundance biomarkers with a broad detection range, which holds great promise as a point-of-care platform for biomedical diagnostics.

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Citations

Jul 15, 2018·Chemistry : a European Journal·Hyeongjoo Choi, Yongwon Jung
Jan 12, 2019·The Analyst·Tingting HongChuanpin Chen
Jul 5, 2019·Advanced Materials·Xiaoxuan ZhangYuanjin Zhao
Oct 9, 2019·The Analyst·Tatsuro NakaoTakehiko Kitamori
May 19, 2020·Biomedical Microdevices·Jacob BergerRashid Bashir
Mar 17, 2020·Analytical and Bioanalytical Chemistry·Rongbin NieLi Yang
Nov 26, 2020·Toxins·Christopher Pöhlmann, Thomas Elßner
Apr 9, 2021·Analytical Chemistry·Zhilong WangBin Li
Jun 18, 2021·Advanced Materials·Xinyi LiuWanwan Li
May 31, 2019·Analytical Chemistry·Lei JiaoChengzhou Zhu

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