Controlled cross-linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Manjula P Mony, Thapasimuthu V Anilkumar

Abstract

Treatment with cross-linking agents for stabilizing biomolecules is an integral step during the preparation of many extracellular matrix-based tissue engineering scaffolds from mammalian organs. However, excess cross-linking may cause nonavailability of biomolecules and consequent deterioration of bioinductive properties of the scaffold. The present study considered controlling the extent of cross-linking in a porcine cholecyst extracellular matrix scaffold prepared by a nonenzymatic and nondetergent method, by ex situ incubation of the source organ in varying concentrations of neutral buffered formaldehyde (10, 4, 1 or 0%; v/v) for in situ cross-linking of biomolecules. Reduction of the formaldehyde concentration resulted in an increase in the extent of biodegradation and a decrease in the compactness of the mesh-like surface microarchitecture of the scaffold. Retention of collagen was maximum when treated with 10% neutral buffered formaldehyde without any variation in the content of elastin and sulphated glycosaminoglycans. Although there was a reduction in the quantity of growth factors following the cross-linking, fibroblasts remained viable on the scaffolds. The retention of major biomolecule was maximum and autodigestion ...Continue Reading

References

Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C H FoxP P Roller
Jan 1, 1970·Virchows Archiv. A: Pathology. Pathologische Anatomie·R D Lillie, P Pizzolato
Dec 1, 1967·The Journal of Clinical Investigation·J D Pstrpw
Nov 26, 2003·The Journal of Biological Chemistry·Bernard MetzWim Jiskoot
Oct 22, 2008·Acta Biomaterialia·Stephen F BadylakThomas W Gilbert
Dec 19, 2008·Chemical Biology & Drug Design·Neha S Gandhi, Ricardo L Mancera
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gregory S Schultz, Annette Wysocki
Apr 29, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joshua D WebsterJosé Ramos-Vara
Jan 20, 2010·Journal of Cellular and Molecular Medicine·Verena M C QuentDietmar W Hutmacher
Apr 30, 2011·Journal of Materials Science. Materials in Medicine·Yilin ZhaoZhongchen Liu
Dec 18, 2013·Nanotechnology·Gregory N GroverKaren L Christman
Mar 7, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Thapasimuthu V AnilkumarAkhila Rajan
Apr 1, 2014·Biochimica Et Biophysica Acta·Marko Fonović, Boris Turk
May 23, 2014·Analytical Chemistry·Julia Kennedy-Darling, Lloyd M Smith
Nov 6, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jaseer MuhamedThapasimuthu V Anilkumar
Aug 13, 2015·Journal of Biomedical Materials Research. Part a·Marta MadaghieleAlessandro Sannino
Nov 8, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jaseer MuhamedThapasimuthu V Anilkumar
Nov 22, 2015·Journal of Biomaterials Applications·Deepa ReviThapasimuthu V Anilkumar
Dec 21, 2016·Acta Biomaterialia·Lisa J WhiteStephen F Badylak
Jan 18, 2018·Toxicologic Pathology·Dhanush Krishna Balakrishnan-NairThapasimuthu Vijayamma Anilkumar

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Citations

Apr 23, 2020·Journal of Biomedical Materials Research. Part a·Reshmi RajThapasimuthu V Anilkumar

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