Controlled delivery of antisense oligodeoxynucleotide from cationically modified phosphorylcholine polymer films

Biomacromolecules
Zhuoqi ZhangJian R Lu

Abstract

Antisense strategy is a promising approach for the prevention of in-stent restenosis if therapeutic agents such as antisense oligodeoxynucleotides (AS-ODNs) can be successfully delivered to the implant site. Optimizing the routes and conditions for controlled loading and release of therapeutic agents from a biocompatible polymer coating is still required. In this study, phosphorylcholine (PC) polymer films bearing different cationic charge densities were deposited onto smooth silicon substrates. The thickness of these films was determined by spectroscopic ellipsometry (SE). Human c-myc AS-ODNs were incorporated into the PC polymer films by immersion in concentrated AS-ODN solution and eluted into PBS under physiological conditions. The elution profile was monitored by UV spectrometry and gel electrophoresis. Cellular uptake of the eluted AS-ODN into vascular smooth muscle cells (VSMCs) was evaluated by fluorescence microscopy. The results showed that ODN loading capacities increased with film thickness and were also strongly dependent on the cationic charge density. AS-ODN release was characterized by a slight initial burst in the first half hour followed by a period of sustained release up to 8 days. Gel electrophoresis demons...Continue Reading

References

Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·T L BurgessT B Kramer
Feb 1, 1995·Circulation Research·E R EdelmanR D Rosenberg
Aug 1, 2000·Colloids and Surfaces. B, Biointerfaces·A L Lewis
Nov 4, 2000·Nature Biotechnology·B D KlugherzR J Levy
Feb 13, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·S HirosueR Langer
May 22, 2002·Journal of the American College of Cardiology·Nicholas N KipshidzeJeffrey W Moses
Jan 24, 2004·Biomaterials·M N V Ravi KumarC M Lehr
Apr 6, 2004·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Nicholas N KipshidzeJeffrey W Moses
Sep 15, 2004·Cardiovascular Research·Shmuel BanaiAlexander Levitzki
Oct 16, 2004·Cardiovascular Research·F SharifT O'Brien
Oct 27, 2004·Journal of Nanoscience and Nanotechnology·Miriam BreunigAchim Goepferich
Feb 16, 2005·Expert Opinion on Biological Therapy·Nicholas KipshidzeDenis Burger
May 4, 2005·Circulation·Marco A Costa, Daniel I Simon
Jul 19, 2005·Orthodontics & Craniofacial Research·P S StaytonA S Hoffman

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Citations

May 11, 2011·Biomacromolecules·Sophie MongeJean-Jacques Robin
Oct 13, 2007·Biomacromolecules·Hironobu TakahashiDavid W Grainger
May 27, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Xiubo ZhaoJikui Luo
Sep 1, 2009·Future Medicinal Chemistry·Steven J P McInnes, Nicolas H Voelcker
Jun 26, 2009·Expert Opinion on Drug Delivery·Xiubo ZhaoJian R Lu
Mar 15, 2011·Colloids and Surfaces. B, Biointerfaces·Yan LiJ R Lu
Jun 20, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhuoqi ZhangJian R Lu
Sep 19, 2007·Journal of Biomedical Materials Research. Part a·K M BalssC A Maryanoff
Dec 3, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Junwu XiaoFangfang Xu
Jul 2, 2009·Journal of Biomedical Materials Research. Part a·Luis A BritoMansoor M Amiji
Oct 13, 2016·Frontiers in Plant Science·Jason KamRattan S Yadav
Nov 24, 2016·Science and Technology of Advanced Materials·Kazuhiko IshiharaYuuki Inoue
May 27, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Xiubo ZhaoJian R Lu
Oct 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Lei YangJian R Lu

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