Controlled delivery of bFGF to recipient bed enhances the vascularization and viability of an ischemic skin flap

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Yuko FujiharaTsuyoshi Takato

Abstract

Therapeutic angiogenesis is a promising approach to treat ischemic skin flaps. We delivered basic fibroblast growth factor (bFGF) to the recipient bed of a rat dorsal skin flap by a drug delivery system with acidic gelatin hydrogel microspheres (AGHMs), and assessed augmentation of neovascularization and flap viability. An axial skin flap was elevated on the back of male Sprague-Dawley rats, and bFGF solution or bFGF-impregnated AGHMs were injected into the recipient bed. The dose of bFGF in the bFGF solution was set to 15 (Sol-15 group), 50 (Sol-50 group), or 150 mug (Sol-150 group). Correspondingly, 2 mg AGHMs were impregnated with 15 (AGHM-15 group), 50 (AGHM-50 group), or 150 mug (AGHM-150 group) bFGF. Other groups of animals received phosphate-buffered saline (Sol-Cont group) or phosphate-buffered saline-impregnated AGHMs (AGHM-Cont group) as controls. Seven days later, analyses of the area of necrosis, microangiographic findings, and histological findings in the flap were carried out. The area of necrosis in the AGHM-150 group was significantly smaller than that in the other groups. Microangiographic and histological analyses showed that neovascularization of the ischemic skin flap significantly increased in the AGHM-150 ...Continue Reading

References

Mar 1, 1992·Annals of Plastic Surgery·M J ImA Fenselau
Feb 1, 1992·Plastic and Reconstructive Surgery·G I Taylor, T Minabe
Jan 1, 1986·Plastic and Reconstructive Surgery·M J Rees, G I Taylor
Jul 1, 1980·Plastic and Reconstructive Surgery·H TsurB Strauch
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E R EdelmanM J Karnovsky
Jan 1, 1997·Plastic and Reconstructive Surgery·S A Syed, R J Restifo
Jun 1, 1997·British Journal of Plastic Surgery·H J WangJ L Chen
Apr 29, 1998·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·A Quirinia, A Viidik
Jul 9, 1998·Plastic and Reconstructive Surgery·K S ZahirJ G Thomson
Mar 26, 1999·Journal of Biomaterials Science. Polymer Edition·Y TabataY Ikada
Jun 6, 2000·Advanced Drug Delivery Reviews·Y Ikada, Y Tabata
Dec 12, 2001·Journal of Reconstructive Microsurgery·M JonesW C Lineaweaver
May 8, 2002·Plastic and Reconstructive Surgery·Przemyslaw LubiatowskiMaria Siemionow
Feb 7, 2004·Blood·Yuichi OikeToshio Suda
May 18, 2005·British Journal of Plastic Surgery·Y FujiharaT Takato

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Citations

Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sabyasachi BiswasChandan K Sen
Jan 1, 2009·Tissue Engineering. Part a·Yuko FujiharaKazuto Hoshi
Mar 19, 2009·Physiological Genomics·Sashwati RoyChandan K Sen
Jul 3, 2013·Pharmacological Reviews·George J ChristKarl-Erik Andersson
Jan 1, 2011·Annals of Vascular Diseases·Mikiko NagayoshiHirokazu Nagawa
Sep 17, 2015·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Tsutomu KashimuraHiroaki Nakazawa
Oct 7, 2008·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Mohammed Ali AkhavaniNorbert Kang
Oct 8, 2014·Plastic and Reconstructive Surgery. Global Open·Shigeru MatsumotoHiroshi Mizuno
Jul 8, 2020·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Masakatsu HiharaKenji Kusumoto
Aug 14, 2019·Advanced Healthcare Materials·Ziyu WangAnthony S Weiss

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