Controlled formation of polylysinized inner pores in injectable microspheres of low molecular weight poly(lactide-co-glycolide) designed for efficient loading of therapeutic cells

Artificial Cells, Nanomedicine, and Biotechnology
Hyeongmin KimJaehwi Lee

Abstract

This study aimed to develop porous microspheres with a suitable porous structure and mechanical property for cell delivery using a comparatively low molecular weight (MW) poly(lactide-co-glycolide) (PLGA) having a weak mechanical strength and fast degradation rate, which could be potentially used for treatment of corneal endothelial diseases. Porous microspheres of 30 kDa PLGA with different pore sizes were prepared by varying preparation conditions, and the microspheres with mean pore diameters approximately 0.5, 1, 2 and 3 times that of a single green fluorescent protein-expressing human embryonic kidney 293 cell, used as a model cell, were chosen for cell loading study. The microspheres with an average pore diameter two times greater than that of the single cell were found to be the most appropriate for efficient cell loading in the inner pore spaces, along with demonstrating a good mechanical property, injectability and biodegradability. To maximize the cell loading amount in the microspheres, the cell adhesive property of the microspheres and cell loading conditions were optimized, leading to approximately 4.2 times increase in the cell loading amount. The porous microspheres designed using the low MW PLGA hold promise as ...Continue Reading

References

Feb 19, 2004·Archives of Pharmacal Research·Yoon Yeo, Kinam Park
Aug 25, 2004·Investigative Ophthalmology & Visual Science·Tatsuya MimuraShiro Amano
Jul 19, 2005·Biomaterials·Taek Kyoung KimTae Gwan Park
Sep 28, 2005·Investigative Ophthalmology & Visual Science·Tatsuya MimuraSatoru Yamagami
Nov 7, 2006·Biochimica Et Biophysica Acta·Dmitry VolodkinHelmuth Mohwald
Mar 22, 2008·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Libero I GiannolaLorenzo Cordone
Aug 12, 2009·Journal of Biological Physics·I O DoagăE Kovács
Feb 16, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Vivian O IkemAlexander Bismarck
May 10, 2011·AAPS PharmSciTech·Francesco CilurzoLuisa Montanari
May 12, 2012·Polymers·Hirenkumar K Makadia, Steven J Siegel
Dec 18, 2013·Acta Biomaterialia·Sílvia J BidarraPedro L Granja
Mar 29, 2014·Journal of Tissue Engineering and Regenerative Medicine·A M LeferinkL Moroni
Jan 13, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Young Sook LeeSung Wan Kim
May 28, 2015·Journal of Biomedical Materials Research. Part a·Mohammed A YassinKamal Mustafa
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Raymond P SeekellBrian D Polizzotti

❮ Previous
Next ❯

Citations

May 1, 2021·Advanced Drug Delivery Reviews·Kamonchanok ThananukulAbdelhamid Elaissari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.