Controlled gene expression with a reverse tetracycline-regulated retroviral vector (RTRV) system

Biochemical and Biophysical Research Communications
T WatsujiM Hagiwara

Abstract

A retroviral vector was constructed with an autoregulatory cassette to allow expression of the gene of interest in response to oral administration of doxycycline (Dox) in vivo. The cassette contains all the components of the reverse tetracycline-regulated (rtTA) system, a drug selectable marker with the internal ribosome entry site and the gene of interest (GFP). FACS analyses showed an induction of GFP-fluorescence of two orders of magnitude in retrovirus-infected 208F cells dependent on the amount of Dox in the medium. Furthermore, oral administration of Dox resulted in GFP expression in transplanted 208F cells in the peritoneal cavity of nude mice. Thus this reverse tetracycline-regulated retroviral vector (RTRV) system simplifies the delivery of controllable genes to cultured and implanted cells. It is hoped that this approach may pave the way to controlled gene expression during a particular window of time in gene therapy applications.

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Oct 1, 1992·Current Opinion in Biotechnology·G T Yarranton
Dec 1, 1991·Experimental Cell Research·M IzumiF Hanaoka
Jul 26, 1990·Nature·M Ptashne, A A Gann
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·P ShockettD G Schatz
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·H OgawaK Umesono
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·A HofmannH M Blau

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Citations

Sep 15, 2000·Human Gene Therapy·P De Felipe, M Izquierdo
Aug 18, 2004·Molecular and Cellular Biology·Kamaleldin E ElagibAdam N Goldfarb
Jul 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chad A EllisGeoffrey J Clark
Jul 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·William PaoHarold E Varmus
Apr 25, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Iranzu GonzálezFernando Lecanda
Jul 2, 2003·Expert Opinion on Biological Therapy·Ulf Müller-LadnerSteffen Gay
Apr 15, 2003·The Journal of Gene Medicine·Anja KnottWolfgang Hillen
Oct 9, 2008·Biochemical and Biophysical Research Communications·Kenji NagaoMitsuo Nishikawa
Nov 2, 2007·International Journal of Cancer. Journal International Du Cancer·Zafira CastañoRuben Pio
Jun 26, 2013·British Journal of Haematology·Cristina BertoloJose A Martinez-Climent
Mar 18, 2015·The Journal of Cell Biology·Howard DonningerGeoffrey J Clark
Aug 14, 1998·Japanese Journal of Cancer Research : Gann·K SugiyamaH Esumi
Nov 18, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·G Daly, Y Chernajovsky
Apr 18, 1998·Somatic Cell and Molecular Genetics·E S KandelI B Roninson
Jun 6, 2008·The Journal of Biological Chemistry·Yoshinobu IchimuraMasaaki Komatsu
Apr 5, 2003·The Journal of Biological Chemistry·Michele D VosGeoffrey J Clark
Jan 28, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xabier Cortés-LavaudMaría D Odero
May 23, 2003·Journal of the American Society of Nephrology : JASN·Hui Y LanRichard J Johnson

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