Controlled Human Malaria Infection with Graded Numbers of Plasmodium falciparum NF135.C10- or NF166.C8-Infected Mosquitoes

The American Journal of Tropical Medicine and Hygiene
Marijke C C LangenbergRobert W Sauerwein

Abstract

Controlled human malaria infections (CHMIs) with Plasmodium falciparum (Pf) parasites are well established. Exposure to five Pf (NF54)-infected Anopheles mosquitoes results in 100% infection rates in malaria-naïve volunteers. Recently Pf clones NF135.C10 and NF166.C8 were generated for application in CHMIs. Here, we tested the clinical infection rates of these clones, using graded numbers of Pf-infected mosquitoes. In a double-blind randomized trial, we exposed 24 malaria-naïve volunteers to bites from one, two, or five mosquitoes infected with NF135.C10 or NF166.C8. The primary endpoint was parasitemia by quantitative polymerase chain reaction. For both strains, bites by five infected mosquitoes resulted in parasitemia in 4/4 volunteers; 3/4 volunteers developed parasitemia after exposure to one or two infected mosquitoes infected with either clone. The prepatent period was 7.25 ± 4.0 days (median ± range). There were no serious adverse events and comparable clinical symptoms between all groups. These data confirm the eligibility of NF135.C10 and NF166.C8 for use in CHMI studies.

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Citations

Mar 14, 2018·Parasites & Vectors·Johanna R OhmMatthew B Thomas
Apr 25, 2019·The American Journal of Tropical Medicine and Hygiene·Annette M SeilieSean C Murphy
May 14, 2020·F1000Research·Nirianne Marie Q Palacpac, Toshihiro Horii
Aug 17, 2019·The Journal of Infectious Diseases·Matthew B LaurensStephen L Hoffman
Apr 22, 2020·The American Journal of Tropical Medicine and Hygiene·Mohamed Farouk ChughlayStephan Chalon
Feb 23, 2020·Seminars in Immunopathology·Mathias SchmalerClaudia Daubenberger
Jan 28, 2021·Expert Review of Vaccines·Danielle I Stanisic, Matthew B B McCall
Mar 7, 2020·The Lancet Infectious Diseases·Marijke C C LangenbergMeta Roestenberg

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Methods Mentioned

BETA
dissecting
PCR

Clinical Trials Mentioned

NCT02149550

Software Mentioned

SPSS

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