Controlled ionic condensation at the surface of a native extremophile membrane

Nanoscale
Sonia Antoranz ConteraJ F Ryan

Abstract

At the nanoscale level biological membranes present a complex interface with the solvent. The functional dynamics and relative flexibility of membrane components together with the presence of specific ionic effects can combine to create exciting new phenomena that challenge traditional theories such as the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory or models interpreting the role of ions in terms of their ability to structure water (structure making/breaking). Here we investigate ionic effects at the surface of a highly charged extremophile membrane composed of a proton pump (bacteriorhodopsin) and archaeal lipids naturally assembled into a 2D crystal. Using amplitude-modulation atomic force microscopy (AM-AFM) in solution, we obtained sub-molecular resolution images of ion-induced surface restructuring of the membrane. We demonstrate the presence of a stiff cationic layer condensed at its extracellular surface. This layer cannot be explained by traditional continuum theories. Dynamic force spectroscopy experiments suggest that it is produced by electrostatic correlation mediated by a Manning-type condensation of ions. In contrast, the cytoplasmic surface is dominated by short-range repulsive hydration forces. These finding...Continue Reading

References

Aug 5, 1978·Journal of Molecular Biology·R HendersonS Whytock
Jan 29, 1976·Nature·L C Gosule, J A Schellman
Jun 8, 1995·Nature·J K Lanyi
Aug 1, 1997·Quarterly Reviews of Biophysics·M G CacaceJ J Ramsden
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·A Oren
Apr 29, 2000·Nature·J Aqvist, V Luzhkov
Sep 14, 2000·Biochimica Et Biophysica Acta·J K Lanyi
Sep 14, 2000·Biochimica Et Biophysica Acta·D J MüllerA Engel
Sep 14, 2000·Biochimica Et Biophysica Acta·N A DencherG Büldt
Aug 11, 2001·Physical Review Letters·A G Moreira, R R Netz
Nov 2, 2001·Nature·S Bernèche, B Roux
Jan 5, 2002·Quarterly Reviews of Biophysics·D Leckband, J Israelachvili
Jan 22, 2002·Physical Review Letters·P M HoffmannA Oral
Nov 1, 2002·Biochimica Et Biophysica Acta·Richard NeutzeEhud M Landau
Jul 17, 2003·Journal of the American Chemical Society·Jonathan N Sachs, Thomas B Woolf
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Florian GarczarekKlaus Gerwert
Mar 24, 2005·Physical Review Letters·Jordi Faraudo, Fernando Bresme
Jan 3, 2006·Biophysical Journal·Kislon VoïtchovskyJ F Ryan
May 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thomas E AngeliniGerard C L Wong
May 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Horia I PetracheV Adrian Parsegian
May 23, 2006·Physical Review Letters·Ohad ZoharUri Sivan
Jun 20, 2006·Biochimica Et Biophysica Acta·Armen Y Mulkidjanian
Dec 22, 2007·Chemical Reviews·Philip Ball
Mar 1, 2008·Science·Douglas J Tobias, John C Hemminger
Dec 19, 2008·Nature·Kenton J Swartz
Apr 28, 2009·Physical Review Letters·Yan Levin
Aug 8, 2009·Nature Nanotechnology·Mingdong DongOzgur Sahin

❮ Previous
Next ❯

Citations

Jul 17, 2014·Nature Communications·Maria RicciKislon Voïtchovsky
Feb 4, 2016·Soft Matter·William TrewbyKislon Voïtchovsky
Jun 2, 2015·Chemical Reviews·Robert HayesRob Atkin
Oct 8, 2016·Nature Communications·Kislon VoïtchovskyMichele Ceriotti
Feb 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zeinab Al-Rekabi, Sonia Contera
Jul 22, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Hongmei BiKislon Voïtchovsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved