Controlled microenvironments to evaluate chemotactic properties of cultured Müller glia

Experimental Eye Research
Juan PenaMaribel Vazquez

Abstract

Emerging therapies have begun to evaluate the abilities of Müller glial cells (MGCs) to protect and/or regenerate neurons following retina injury. The migration of donor cells is central to many reparative strategies, where cells must achieve appropriate positioning to facilitate localized repair. Although chemical cues have been implicated in the MGC migratory responses of numerous retinopathies, MGC-based therapies have yet to explore the extent to which external biochemical stimuli can direct MGC behavior. The current study uses a microfluidics-based assay to evaluate the migration of cultured rMC-1 cells (as model MGC) in response to quantitatively-controlled microenvironments of signaling factors implicated in retinal regeneration: basic Fibroblast Growth factor (bFGF or FGF2); Fibroblast Growth factor 8 (FGF8); Vascular Endothelial Growth Factor (VEGF); and Epidermal Growth Factor (EGF). Findings indicate that rMC-1 cells exhibited minimal motility in response to FGF2, FGF8 and VEGF, but highly-directional migration in response to EGF. Further, the responses were blocked by inhibitors of EGF-R and of the MAPK signaling pathway. Significantly, microfluidics data demonstrate that changes in the EGF gradient (i.e. change in ...Continue Reading

References

Aug 22, 1993·The Journal of Comparative Neurology·M F HumphreyS Wiffen
Sep 1, 1996·Journal of Lipid Mediators and Cell Signalling·E MeuilletH Dreyfus
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sotaro OotoMasayo Takahashi
Nov 23, 2005·Journal of Biomedical Materials Research. Part a·Nathan R F BeeleySigne E Varner
Feb 21, 2006·The British Journal of Ophthalmology·H A Quigley, A T Broman
Jul 15, 2006·Progress in Retinal and Eye Research·Andreas BringmannAndreas Reichenbach
Sep 15, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jan H SchefeHeiko Funke-Kaiser
Nov 24, 2006·Investigative Ophthalmology & Visual Science·Guoliang JiangSylvia B Smith
Apr 15, 2008·Investigative Ophthalmology & Visual Science·Natalie D BullKeith R Martin
May 20, 2008·Investigative Ophthalmology & Visual Science·Jung Eun LeeWai T Wong
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Nov 6, 2009·Cellular and Molecular Bioengineering·Diana YuGabriel A Silva
Dec 15, 2010·Journal of Biomechanical Engineering·Qingjun KongMaribel Vazquez
Feb 16, 2012·Nature Communications·Marie VersaevelSylvain Gabriele
Oct 6, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·William J Ashby, Andries Zijlstra
May 4, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pablo F BarcelonaGustavo A Chiabrando
May 29, 2013·Lab on a Chip·Jiandong WuFrancis Lin
Nov 26, 2013·Cellular and Molecular Bioengineering·Veronica DuduMaribel Vazquez
Jun 1, 2012·Cellular and Molecular Bioengineering·Richard A AbleMaribel Vazquez
Jan 15, 2014·Progress in Retinal and Eye Research·Jenny R Lenkowski, Pamela A Raymond
Mar 14, 2014·Nature·Eric K SackmannDavid J Beebe
Mar 31, 2015·PloS One·Barbara LorberKeith R Martin
Apr 15, 2015·Frontiers in Bioengineering and Biotechnology·Sébastien G RicoultDavid Juncker
Sep 29, 2015·Expert Opinion on Biological Therapy·Henry Klassen
Oct 18, 2015·Biomedical Microdevices·Shawn MishraMaribel Vazquez
Oct 30, 2015·Investigative Ophthalmology & Visual Science·Valeria E LorencMaría C Sánchez
Nov 26, 2015·Cellular and Molecular Bioengineering·Jennifer Rico-VarelaMaribel Vazquez
Nov 27, 2015·Experimental Eye Research·Lucia GaddiniFiorella Malchiodi-Albedi

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.