Controlled microfluidic encapsulation of cells, proteins, and microbeads in lipid vesicles

Journal of the American Chemical Society
Yung-Chieh TanAbraham Phillip Lee

Abstract

Cells have been encapsulated inside lipid vesicles by using a new microfluidic lipid vesicle formulation technique. Lipid vesicles are formulated within minutes without using toxic lipid solvents. The encapsulation efficiency inside the vesicles is controlled by the microfluidic flows. Green fluorescent proteins (GFP), carcinoma cells, and bead encapsulated vesicles have mean diameters of 27.2 mum, 62.4 mum, and 55.9 mum, respectively. The variations of vesicle sizes are approximately 20% for the GFP and cell encapsulated vesicles and approximately 10% for the bead encapsulated vesicles.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Nov 25, 2000·Biochimica Et Biophysica Acta·M OllivonM Paternostre
May 1, 2001·Physical Review Letters·T ThorsenS R Quake
Mar 14, 2002·Electrophoresis·Laurie E LocascioMichael Gaitan
Aug 23, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Andreas WagnerHermann Katinger
Nov 15, 2003·Analytical and Bioanalytical Chemistry·Soohyoun Ahn-YoonRichard A Durst
Mar 5, 2004·Journal of the American Chemical Society·Andreas JahnLaurie E Locascio
Jul 14, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Marc MichelVincent Ball
Dec 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Vincent Noireaux, Albert Libchaber
Feb 22, 2005·Methods in Enzymology·Isam I SalemNejat Düzgüneş
Jul 20, 2005·Lab on a Chip·Natalya V ZaytsevaAntje J Baeumner
Oct 14, 2005·Journal of the American Chemical Society·Bo ZhengRustem F Ismagilov

❮ Previous
Next ❯

Citations

Mar 13, 2008·Analytical and Bioanalytical Chemistry·Hiroaki Suzuki, Shoji Takeuchi
Oct 28, 2010·Biomedical Microdevices·Zhongcheng GongLong Que
Sep 3, 2011·Systems and Synthetic Biology·James SmaldonNatalio Krasnogor
Mar 28, 2008·Analytical Chemistry·Jennifer C GaddDaniel T Chiu
Apr 1, 2011·ACS Applied Materials & Interfaces·Peichi C HuNoah Malmstadt
Dec 18, 2012·Biomacromolecules·Hua WeiSuzie H Pun
Dec 1, 2006·The Journal of Physical Chemistry. B·Christopher L KuyperDaniel T Chiu
Feb 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Salil P DesaiJoel Voldman
Mar 29, 2007·Lab on a Chip·Christopher E Sims, Nancy L Allbritton
Mar 16, 2007·Chemical Communications : Chem Comm·A HuebnerJ B Edel
Jan 31, 2008·Lab on a Chip·Shia-Yen TehAbraham P Lee
Jul 25, 2008·Lab on a Chip·Ansgar HuebnerAndrew J Demello
Jun 18, 2009·Lab on a Chip·Su-Kyoung ChaeJi Yoon Kang
Jan 8, 2013·Lab on a Chip·Dirk van Swaay, Andrew deMello
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Max Chabert, Jean-Louis Viovy
Jun 28, 2008·Journal of the Royal Society, Interface·Hsuan-Hong LaiNancy L Allbritton
Feb 21, 2008·The European Physical Journal. E, Soft Matter·F MalloggiF Mugele
Nov 1, 2012·Sensors·Byeong Il KimChang-Soo Lee
Aug 7, 2009·The Review of Scientific Instruments·Zahra NorooziMarc Madou
Jun 5, 2012·Biomicrofluidics·Ho Cheung ShumDavid A Weitz
Aug 2, 2013·The Journal of Chemical Physics·Stephen Mirigian, Murugappan Muthukumar
Jun 2, 2012·Biomicrofluidics·Robert LinAbraham P Lee
Nov 14, 2013·Chemistry and Physics of Lipids·Yogita P Patil, Sameer Jadhav
Sep 4, 2007·The Journal of Chemical Physics·Kurt A SmithAnna C Balazs
Jul 1, 2010·Expert Opinion on Drug Discovery·Cornelia CarstensHolger Becker
Nov 1, 2008·Expert Opinion on Drug Discovery·Richard F WinkleSanjiv Sharma
Dec 1, 2007·Expert Opinion on Drug Discovery·Bong Geun ChungAli Khademhosseini
Nov 1, 2010·Expert Opinion on Drug Discovery·Dario Lombardi, Petra S Dittrich
Jan 22, 2013·Colloids and Surfaces. B, Biointerfaces·M MijajlovicM J Biggs
Feb 24, 2016·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Rishad R JivaniBhagyesh P Jhala
Nov 19, 2015·Advanced Drug Delivery Reviews·Yvonne PerrieVinod Nadella
Sep 25, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Senthil ArumugamPetra Schwille
Jul 24, 2015·ACS Chemical Biology·Wei LuoMuhammad N Yousaf
Jun 1, 2016·Journal of the American Chemical Society·Nan-Nan DengWilhelm T S Huck
Aug 9, 2016·Interface Focus·Chiara Martino, Andrew J deMello
Dec 17, 2016·Journal of the American Chemical Society·Nan-Nan DengWilhelm T S Huck

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Langmuir : the ACS Journal of Surfaces and Colloids
Ho Cheung ShumD A Weitz
Journal of the American Chemical Society
Sandro Matosevic, Brian M Paegel
Langmuir : the ACS Journal of Surfaces and Colloids
Andreas JahnMichael Gaitan
© 2022 Meta ULC. All rights reserved