Controlled Nanoscale Topographies for Osteogenic Differentiation of Mesenchymal Stem Cells

ACS Applied Materials & Interfaces
Catarina R PedrosaMarie-Christine Durrieu

Abstract

Nanotopography with length scales of the order of extracellular matrix elements offers the possibility of regulating cell behavior. Investigation of the impact of nanotopography on cell response has been limited by the inability to precisely control geometries, especially at high spatial resolutions and across practically large areas. In this paper, we demonstrate well-controlled and periodic nanopillar arrays of silicon and investigate their impact on osteogenic differentiation of human mesenchymal stem cells (hMSCs). Silicon nanopillar arrays with critical dimensions in the range of 40-200 nm, exhibiting standard deviations below 15% across full wafers, were realized using the self-assembly of block copolymer colloids. Immunofluorescence and quantitative polymerase chain reaction measurements reveal clear dependence of osteogenic differentiation of hMSCs on the diameter and periodicity of the arrays. Further, the differentiation of hMSCs was found to be dependent on the age of the donor. While osteoblastic differentiation was found to be promoted by the pillars with larger diameters and heights independent of donor age, they were found to be different for different spacings. Pillar arrays with smaller pitch promoted different...Continue Reading

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Citations

Jun 14, 2020·Journal of Functional Biomaterials·Giuseppe ArrabitoBruno Pignataro
May 23, 2020·International Journal of Nanomedicine·Changhong ZhaoXiaoyuan Lu
Apr 21, 2021·Journal of Nanoscience and Nanotechnology·Usama TahirMyung Yung Jeong
Jul 14, 2021·Journal of Biomedical Materials Research. Part a·Nianfang MaJonathan T Butcher

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