Controlled ovarian stimulation and progesterone supplementation affect vaginal and endometrial microbiota in IVF cycles: a pilot study.

Journal of Assisted Reproduction and Genetics
Andrea CarossoChiara Benedetto

Abstract

Does controlled ovarian stimulation (COS) and progesterone (P) luteal supplementation modify the vaginal and endometrial microbiota of women undergoing in vitro fertilization? Fifteen women underwent microbiota analysis at two time points: during a mock transfer performed in the luteal phase of the cycle preceding COS, and at the time of fresh embryo transfer (ET). A vaginal swab and the distal extremity of the ET catheter tip were analyzed using next-generation 16SrRNA gene sequencing. Heterogeneity of the bacterial microbiota was assessed according to both the Bray-Curtis similarity index and the Shannon diversity index. Lactobacillus was the most prevalent genus in the vaginal samples, although its relative proportion was reduced by COS plus P supplementation (71.5 ± 40.6% vs. 61.1 ± 44.2%). In the vagina, an increase in pathogenic species was observed, involving Prevotella (3.5 ± 8.9% vs. 12.0 ± 19.4%), and Escherichia coli-Shigella spp. (1.4 ± 5.6% vs. 2.0 ± 7.8%). In the endometrium, the proportion of Lactobacilli slightly decreased (27.4 ± 34.5% vs. 25.0 ± 29.9%); differently, both Prevotella and Atopobium increased (3.4 ± 9.5% vs. 4.7 ± 7.4% and 0.7 ± 1.5% vs. 5.8 ± 12.0%). In both sites, biodiversity was greater after ...Continue Reading

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Citations

Apr 14, 2021·ACS Infectious Diseases·Fulvio BorellaChiara Benedetto
Apr 27, 2021·Current Opinion in Obstetrics & Gynecology·Lidia Lorite AguileraIgnacio Cristóbal Quevedo
Jun 11, 2021·Journal of Assisted Reproduction and Genetics·Paula Punzón-Jiménez, Elena Labarta
Aug 17, 2021·Bioscience Reports·Karen GrewalPhillip R Bennett

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Datasets Mentioned

BETA
PRJNA634237

Methods Mentioned

BETA
PCR

Software Mentioned

Labdsv®
SmartSeq
MicrobAT® Microbiota Analysis Tool
RAM
Vegan®
RColorBrewer®
ANOSIM
R
Heatplus®
Ggplot2®

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