Controlled release of FK506 from micropatterned PLGA films: potential for application in peripheral nerve repair

Neural Regeneration Research
Brett DavisJayant Agarwal

Abstract

After decades of research, peripheral nerve injury and repair still frequently results in paralysis, chronic pain and neuropathies leading to severe disability in patients. Current clinically available nerve conduits only provide crude guidance of regenerating axons across nerve gap without additional functionality. FK506 (Tacrolimus), an FDA approved immunosuppressant, has been shown to enhance peripheral nerve regeneration but carries harsh side-effects when delivered systemically. The objective of this study was to develop and evaluate a bioresorbable drug delivery system capable of local extended delivery of FK506 that also provides topological guidance cues to guide axon growth via microgrooves. Photolithography was used to create micropatterned poly(lactide-co-glycolic acid) (PLGA) films embedded with FK506. Non-patterned, 10/10 μm (ridge/groove width), and 30/30 μm patterned films loaded with 0, 1, and 3 μg/cm2 FK506 were manufactured and characterized. In vitro FK506 rate of release testing indicated that the films are capable of an extended (at least 56 days), controlled, and scalable release of FK506. Neurite extension bioactivity assay indicated that FK506 released from the films (concentration of samples tested rang...Continue Reading

References

Dec 12, 1983·Brain Research·C IdeS Onodera
Feb 11, 1998·Molecular Neurobiology·B G Gold
Jun 8, 1999·Plastic and Reconstructive Surgery·V B Doolabh, S E Mackinnon
Jun 24, 1999·Journal of Biomedical Materials Research·L LuA G Mikos
Aug 22, 2000·The Journal of the American Academy of Orthopaedic Surgeons·S K Lee, S W Wolfe
Jul 27, 2001·Current Opinion in Chemical Biology·O Pillai, R Panchagnula
Dec 26, 2001·Chemical Reviews·K E UhrichK M Shakesheff
May 4, 2002·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·N FaisantJ P Benoit
Sep 5, 2006·Neuroreport·Simon AtkinsPeter P Robinson
Jul 24, 2007·Progress in Neurobiology·X NavarroAntoni Valero-Cabré
Oct 7, 2008·Acta Biomaterialia·Li YaoAbhay Pandit
Feb 17, 2009·Clinical Journal of the American Society of Nephrology : CJASN·Maarten NaesensMinnie Sarwal
May 5, 2010·Annual Review of Biomedical Engineering·Diane Hoffman-KimRavi V Bellamkonda
May 24, 2011·Foot and Ankle Clinics·Victoria R Masear
Feb 1, 2012·Neurorehabilitation and Neural Repair·Ying YanPhilip J Johnson
May 12, 2012·Polymers·Hirenkumar K Makadia, Steven J Siegel
Jul 26, 2012·Journal of Biomedical Materials Research. Part a·Wen-Ta SuYu-Yin Shih
Oct 30, 2012·The Journal of Hand Surgery·Mickey S ChoGregory M Buncke
Jan 11, 2013·Journal of Reconstructive Microsurgery·P Konofaos, J P Ver Halen
Oct 3, 2014·BioMed Research International·D Grinsell, C P Keating
Feb 11, 2015·Orthopedics·Zinon T KokkalisPanayotis N Soucacos
Apr 9, 2015·Biotechnology and Bioengineering·Kasra TajdaranGregory H Borschel
May 20, 2016·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·A Y MekajY H Mekaj
Aug 10, 2016·Muscle & Nerve·Pratima LabrooJayant Agarwal
Aug 23, 2017·Journal of Neural Engineering·Pratima LabrooJay Agarwal

❮ Previous
Next ❯

Citations

Oct 3, 2019·Expert Review of Clinical Pharmacology·T M SaffariC A Hundepool
Dec 12, 2019·Frontiers in Bioengineering and Biotechnology·Cristiana R CarvalhoRui L Reis
Jan 30, 2021·Microsurgery·Anthony DeLeonibusBahar Bassiri Gharb
Apr 30, 2021·Biotechnology and Bioengineering·Tiam M SaffariAlexander Y Shin

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy
ELISA

Software Mentioned

Excel
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.