Controlled release of glial cell line-derived neurotrophic factor from poly(ε-caprolactone) microspheres

Drug Delivery and Translational Research
Andrew AgbayStephanie Michelle Willerth

Abstract

Glial cell line-derived neurotrophic factor (GDNF), a growth factor expressed in the central nervous system, promotes the survival of both dopaminergic and motor neurons, making it a promising candidate for neurodegenerative disease therapy. Although GDNF is currently being evaluated in clinical trials for the treatment of Parkinson's disease (PD), the current delivery method using catheter implantation has certain limitations in terms of delivering GDNF safely and effectively. As a proof of concept, we encapsulated GDNF into poly(ε-caprolactone) (PCL) microspheres to enable controlled drug release for 25 days. First, microspheres were loaded with bovine serum albumin (BSA) to determine the optimal fabrication conditions necessary to achieve the desired release rates of protein. BSA was then used as a carrier protein to preserve GDNF activity during the fabrication process in the presence of organic solvents. GDNF-encapsulated microspheres were created and characterized using scanning electron microscopy. Next, the in vitro release of GDNF along with microsphere morphology was tracked over 25 days. Finally, the bioactivity of the released GDNF was confirmed using PC12 cells. This work demonstrates the potential of such microsph...Continue Reading

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Citations

Dec 17, 2014·Pharmaceutical Development and Technology·Jakub VysloužilKateřina Dvořáčková
Sep 25, 2015·Journal of Biomedical Materials Research. Part a·Kasra TajdaranGregory H Borschel
Oct 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·N K MohtaramS M Willerth
Aug 8, 2017·Biosensors·Azra RajwaniStephanie M Willerth
Mar 27, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alejandro ForiguaKatherine S Elvira

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