Controlled respiratory gas delivery to embryonic renal epithelial explants in perfusion culture

Tissue Engineering
R StrehlW W Minuth

Abstract

During generation of artificial tissues high levels of oxygen are usually available whereas after implantation into a recipient's body the implant is not vascularized immediately, which leads to low oxygen partial pressures within the implanted tissue. Under these conditions cells will experience an oxygen shortage, contrasting with the abundance of oxygen during culture. It is uncertain whether tissues can be trained to tolerate such an acute hypoxic situation so that nonphysiological stress reactions and tissue necrosis can be avoided. To investigate the effects of varying oxygen levels on embryonic renal tissue in vitro we have been developing a model system combining continuous medium renewal with the ability to control levels of oxygen and carbon dioxide by gas equilibration through gas-permeable tubing. Renal embryonic tissue from neonatal rabbit was cultured in serum-free Iscove's modified Dulbecco's medium at 45, 90, 115, and 160 mmHg oxygen partial pressure for 14 days under continuous medium exchange in such a setup. After a 14-day culture period tissue sections were analyzed by cell biological methods and compared with fresh tissue histology. Surprisingly, embryonic renal explants survive and maintain good morphology...Continue Reading

References

Jan 1, 1990·Journal of Chemical Technology and Biotechnology·T ManoT Kobayashi
May 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S A Suleiman, J B Stevens
Jan 1, 1994·Advances in Experimental Medicine and Biology·G F Ye, S F Silverton
Jul 1, 1993·The American Journal of the Medical Sciences·T Helfman, V Falanga
Jan 1, 1997·Cell and Tissue Research·S KlothW W Minuth
Apr 1, 1997·Neurochemical Research·J K YunE G Lapetina
Jan 8, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S W O'DriscollC N Commisso
Apr 9, 1998·Transplantation Proceedings·A HyderJ Schrezenmeir
Jul 27, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·J D Davidson, T A Mustoe
Nov 24, 2001·Respiration Physiology·M Erecińska, I A Silver
Jun 22, 2002·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Karl SchumacherWill W Minuth
Oct 22, 2003·Journal of the American Society of Nephrology : JASN·Karl SchumacherWill W Minuth

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Citations

Mar 21, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Martin HammerDietrich Schweitzer
Jan 26, 2010·Biomaterials·Will W MinuthAnne Glashauser
Sep 27, 2005·Journal of Biotechnology·Stephanie Nagel-HeyerRalf Pörtner
Feb 2, 2013·Clinical and Translational Medicine·Will W Minuth, Lucia Denk

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