Apr 23, 2020

The SEM-4 transcription factor is required for regulation of the oxidative stress response in Caenorhabditis elegans

BioRxiv : the Preprint Server for Biology
A. RafikovaTerrance J. Kubiseski


Oxidative stress causes damage to cells by creating reactive oxygen species (ROS) and the overproduction of ROS have been linked to the onset of premature ageing. We previously found that a brap-2 (BRCA1 associated protein 2) mutant significantly increases the expression of phase II detoxification enzymes in C. elegans. An RNAi suppression screen to identify transcription factors involved in the production of gst-4 mRNA in brap-2 worms identified SEM-4 as a potential candidate. Here, we show that knockdown of sem-4 suppresses the activation of gst-4 caused by the mutation in brap-2. We also demonstrate that sem-4 is required for survival upon exposure to oxidative stress and that SEM-4 is required for expression of the transcription factor SKN-1C. These findings identify a novel role for SEM-4 in ROS detoxification by regulating expression of SKN-1C and the phase II detoxification genes.

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Mentioned in this Paper

Genome-Wide Association Study
Meta-Analysis (Publications)
Meta Analysis (Statistical Procedure)
Protein Methylation
CTSE gene

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