Controlling DNA orientation on mixed ssDNA/OEG SAMs

Langmuir : the ACS Journal of Surfaces and Colloids
Christina BoozerShaoyi Jiang

Abstract

We present and characterize a mixed self-assembled monolayer (SAM) consisting of single-stranded oligonucleotide (ssDNA)- and oligo(ethylene glycol) (OEG)-terminated thiols. The ssDNA/OEG SAMs are prepared by simultaneous coadsorption from a common thiol solution over a broad range of compositions. Electron spectroscopy for chemical analysis (ESCA) is used to measure the surface coverage of ssDNA, whereas surface plasmon resonance (SPR) sensor is used to measure the hybridization of complementary ssDNA and protein resistance. Through the complementary use of these techniques, we find that the composition of OEG in the assembly solution controls a key parameter: the surface coverage of ssDNA on the surface. There is evidence that it influences the orientation of the immobilized ssDNA probes. Lower OEG concentrations yield a surface with higher ssDNA coverage and less favorable orientation, whereas higher OEG concentrations produce a surface with lower DNA coverage and more favorable orientation. Competition between these two effects controls the hybridization efficiency of the ssDNA surface. Compared to ssDNA surfaces prepared with other diluent thiols, the use of OEG improves the protein resistance of the surface, making it mor...Continue Reading

References

May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A C PeaseS P Fodor
May 20, 1998·Nature Biotechnology·J M Gower
Dec 9, 1998·Analytical Chemistry·A B SteelM J Tarlov
Jun 24, 2000·Nature·D J Lockhart, E A Winzeler
Apr 24, 2003·Journal of the American Chemical Society·Dmitri Y PetrovykhMichael J Tarlov
Sep 8, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Jon LaddShaoyi Jiang

❮ Previous
Next ❯

Citations

Apr 8, 2011·Analytical and Bioanalytical Chemistry·Sanna AuerInger Vikholm-Lundin
Apr 9, 2013·Analytica Chimica Acta·Hana Šípová, Jiří Homola
Mar 1, 2012·Chemical Reviews·Emil Paleček, Martin Bartošík
Dec 14, 2011·Journal of the American Chemical Society·Jian LiangGiacinto Scoles
Jun 19, 2012·Journal of the American Chemical Society·Seong Jung Kwon, Allen J Bard
Jun 10, 2010·Physical Chemistry Chemical Physics : PCCP·Stefan ZornFrank Schreiber
Aug 1, 2013·The Journal of Physical Chemistry. B·M Nuruzzaman KhanMichael Zharnikov
Jun 30, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Christoph H-H TraulsenChristoph A Schalley
Jan 14, 2011·Biosensors & Bioelectronics·Xiaojuan Zhang, Vamsi K Yadavalli
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Apr 17, 2015·Biosensors & Bioelectronics·Ahmed M DebelaCiara K O'Sullivan
Dec 4, 2014·Colloids and Surfaces. B, Biointerfaces·Yanli YangBo Liedberg
Jun 3, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Youlong Ma, Matthew Libera
Sep 21, 2013·International Journal of Legal Medicine·Michael Krawczak, Jürgen Goebel
Jan 24, 2019·International Journal of Molecular Sciences·Susana CampuzanoJosé M Pingarrón
May 30, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Siyoung Choi, William L Murphy
Dec 12, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Crissy L RhodesStefan Franzen
Sep 14, 2017·Chemical Reviews·Shuaidi ZhangVladimir V Tsukruk
Nov 15, 2014·Analytical Chemistry·Stephen M WooLeyla Soleymani
Mar 9, 2018·ACS Applied Materials & Interfaces·Anna HerrmannUwe Schedler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.