Controlling Enzymatic Polymerization from Surfaces with Switchable Bioaffinity

Biomacromolecules
Mohammad DivandariEdmondo M Benetti

Abstract

The affinity of surfaces toward proteins is found to be a key parameter to govern the synthesis of polymer brushes by surface-initiated biocatalytic atom transfer radical polymerization (SI-bioATRP). While the "ATRPase" hemoglobin (Hb) stimulates only a relatively slow growth of protein repellent brushes, the synthesis of thermoresponsive grafts can be regulated by switching the polymer's attraction toward proteins across its lower critical solution temperature (LCST). Poly(N-isopropylacrylamide) (PNIPAM) brushes are synthesized in discrete steps of thickness at temperatures above LCST, while the biocatalyst layer is refreshed at T < LCST. Multistep surface-initiated biocatalytic ATRP demonstrates a high degree of control, results in high chain end group fidelity and enables the synthesis of multiblock copolymer brushes under fully aqueous conditions. The activity of Hb can be further modulated by tuning the accessibility of the heme pocket within the protein. Hence, the multistep polymerization is accelerated at acid pH, where the enzyme undergoes a transition from its native to a molten globule conformation. The controlled synthesis of polymer brushes by multistep SI-bioATRP highlights how a biocatalytic synthesis of grafted ...Continue Reading

References

Jan 23, 2004·Chemical Society Reviews·Steve EdmondsonWilhelm T S Huck
Aug 19, 2004·Journal of Agricultural and Food Chemistry·Hordur G Kristinsson, Herbert O Hultin
Feb 7, 2006·Biomaterials·Didier FalconnetMarcus Textor
Feb 22, 1991·Science·S T Milner
Aug 13, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Zengwei GuoPeter Walde
Sep 22, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Sina BurkertManfred Stamm
Feb 23, 2010·Antioxidants & Redox Signaling·Brandon J Reeder
May 10, 2011·Chemical Communications : Chem Comm·Yeap-Hung NgChristina L L Chai
Aug 13, 2011·Current Opinion in Chemical Biology·Peter KingshottHans J Griesser
Oct 17, 2012·Biosensors & Bioelectronics·Ying WanChunhai Fan
Dec 1, 2012·Analytical Chemistry·Vinalia TjongAshutosh Chilkoti
Jun 7, 2013·Biomacromolecules·Tilana B SilvaNico Bruns
Oct 12, 2013·Angewandte Chemie·Antonina SimakovaKrzysztof Matyjaszewski
Jan 17, 2015·Sensors·Erik Reimhult, Fredrik Höök
Jan 27, 2015·Macromolecular Rapid Communications·Maria Valentina DinuNico Bruns
Jan 23, 2016·Chemical Reviews·Shin-ichiro ShodaShiro Kobayashi
Feb 6, 2016·International Journal of Molecular Sciences·Scott R ZavadaTimothy F Scott
Mar 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guosong WuPaul K Chu

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