Abstract
Living systems are capable of locomotion, reconfiguration and replication. To perform these tasks, cells spatiotemporally coordinate the interactions of force-generating, 'active' molecules that create and manipulate non-equilibrium structures and force fields of up to millimetre length scales1-3. Experimental active-matter systems of biological or synthetic molecules are capable of spontaneously organizing into structures4,5 and generating global flows6-9. However, these experimental systems lack the spatiotemporal control found in cells, limiting their utility for studying non-equilibrium phenomena and bioinspired engineering. Here we uncover non-equilibrium phenomena and principles of boundary-mediated control by optically modulating structures and fluid flow in an engineered system of active biomolecules. Our system consists of purified microtubules and light-activatable motor proteins that crosslink and organize the microtubules into distinct structures upon illumination. We develop basic operations-defined as sets of light patterns-to create, move and merge the microtubule structures. By combining these operations, we create microtubule networks that span several hundred micrometres in length and contract at speeds up to ...Continue Reading
References
Sep 30, 1994·Science·W E Theurkauf
Sep 26, 1997·Nature·F J NédélecS Leibler
Apr 6, 2001·Physical Review Letters·F NédélecA C Maggs
May 12, 2001·Science·T SurreyE Karsenti
Dec 12, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·H Y Lee, M Kardar
Aug 19, 2007·PLoS Biology·Orion D WeinerMarc W Kirschner
Mar 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Raymond E GoldsteinJan-Willem van de Meent
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Margaret L GardelClare M Waterman
Jan 15, 2011·Physical Review Letters·Knut DrescherIdan Tuval
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tamar ShinarMichael J Shelley
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Knut DrescherRaymond E Goldstein
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sujoy GangulyRaymond E Goldstein
Nov 9, 2012·Nature·Tim SanchezZvonimir Dogic
Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Susumu AoyamaYuichi Hiratsuka
Nov 10, 2013·Nature·Antoine BricardDenis Bartolo
Nov 16, 2013·Science·Matthew C GoodRebecca Heald
Mar 5, 2014·Nature·Bing HeEric Wieschaus
Sep 6, 2014·Science·Felix C KeberAndreas R Bausch
Nov 5, 2014·Molecular Biology of the Cell·Sophie Dumont, Manu Prakash
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gurkan GuntasBrian Kuhlman
Aug 19, 2015·Nature Materials·Stephen J DeCampZvonimir Dogic
Dec 25, 2015·ELife·Peter J FosterDaniel J Needleman
Oct 16, 2016·Nature Communications·Matthias SchupplerAndreas R Bausch
Mar 25, 2017·Science·Kun-Ta WuZvonimir Dogic
Sep 29, 2017·Molecular Systems Biology·Julio M BelmonteFrançois Nédélec
Feb 7, 2018·Nature Cell Biology·Matthäus MittaschMoritz Kreysing
Citations
Nov 7, 2019·Nature·Geoffrey Belknap
Mar 20, 2020·ELife·Pooja SureshSophie Dumont
May 28, 2020·Advanced Biosystems·Djamel Eddine ChafaiPavel Dráber
Sep 25, 2020·Nature Communications·Arno van der WeijdenPeter A Korevaar
Nov 22, 2020·Soft Matter·Zhengyang ZhouPengyu Hong
Feb 7, 2021·Science Advances·Gloria LeeRae M Robertson-Anderson
Nov 18, 2020·Nature Chemistry·Caleb H MeredithLauren D Zarzar
Jan 29, 2021·Frontiers in Immunology·Falk SchneiderMarco Fritzsche
Feb 20, 2021·Nature Chemical Biology·Paul V RuijgrokZev Bryant
Feb 20, 2021·Nature Materials·Rui ZhangJuan J de Pablo
Apr 6, 2021·Computational and Structural Biotechnology Journal·Jiří PrůšaMichal Cifra
Apr 21, 2021·Nature Materials·Takahiro NittaYuichi Hiratsuka
Apr 25, 2021·Mathematical Biosciences and Engineering : MBE·Y -N YoungDavid B Stein
May 15, 2021·ACS Synthetic Biology·Jessica G BermudezMatthew C Good
Jul 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Alexandra M TayarZvonimir Dogic