Controlling the orientation and synaptic differentiation of myotubes with micropatterned substrates.

Biophysical Journal
Jacinthe GingrasJoshua R Sanes

Abstract

Micropatterned poly(dimethylsiloxane) substrates fabricated by soft lithography led to large-scale orientation of myoblasts in culture, thereby controlling the orientation of the myotubes they formed. Fusion occurred on many chemically identical surfaces in which varying structures were arranged in square or hexagonal lattices, but only a subset of patterned surfaces yielded aligned myotubes. Remarkably, on some substrates, large populations of myotubes oriented at a reproducible acute angle to the lattice of patterned features. A simple geometrical model predicts the angle and extent of orientation based on maximizing the contact area between the myoblasts and patterned topographic surfaces. Micropatterned substrates also provided short-range cues that influenced higher-order functions such as the localization of focal adhesions and accumulation of postsynaptic acetylcholine receptors. Our results represent what we believe is a new approach for musculoskeletal tissue engineering, and our model sheds light on mechanisms of myotube alignment in vivo.

References

Nov 15, 1985·Science·H M BlauC Webster
Apr 15, 1999·Annual Review of Neuroscience·J R Sanes, J W Lichtman
Dec 30, 1999·Biomaterials·R S KaneG M Whitesides
Feb 19, 2002·Chemical Reviews·Younan XiaGeorge M. Whitesides
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·P ClarkM Peckham
Mar 24, 2004·The Journal of Cell Biology·Terrance T KummerJoshua R Sanes
Jul 17, 2004·Annual Review of Biomedical Engineering·Christopher S ChenJoe Tien
Oct 14, 2004·The Journal of Cell Biology·Jennifer R MorganPietro De Camilli
Mar 15, 2005·Connective Tissue Research·Solfrid ArnesenMoira Lawson
Jan 3, 2006·Current Opinion in Neurobiology·Terrance T KummerJoshua R Sanes
Mar 9, 2006·Nano Letters·Ngan F HuangSong Li
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Julie A Champion, Samir Mitragotri
Aug 10, 2007·Journal of Biomedical Materials Research. Part a·S A RiboldiS Mantero
Apr 1, 2008·Pharmaceutical Research·Julie A ChampionSamir Mitragotri
Jun 7, 2008·Circulation Research·Kris Noel DahlJan Lammerding
Jun 25, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Daniel L YamamotoTore Bengtsson
Sep 17, 2008·The Journal of Cell Biology·Hiroshi NishimuneJoshua R Sanes
Oct 30, 2008·Biotechnology and Bioengineering·Yi ZhaoSudha Agarwal

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Citations

Sep 11, 2012·Biomedical Microdevices·Leonardo Ricotti, Arianna Menciassi
Jan 1, 2013·ACS Applied Materials & Interfaces·Francesco GrecoVirgilio Mattoli
May 26, 2011·Tissue Engineering. Part a·Cristian Pablo PennisiVladimir Zachar
May 23, 2012·Tissue Engineering. Part a·Claire MongeCatherine Picart
Jun 12, 2014·HNO·J Stern-Straeter, K Hörmann
Aug 28, 2012·Advanced Drug Delivery Reviews·Hong Nam KimKahp-Yang Suh
Sep 12, 2012·Acta Biomaterialia·James M DuganStephen J Eichhorn
Nov 26, 2010·Journal of Cellular and Molecular Medicine·Dorothee KlumppJustus P Beier
Dec 10, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Rebecca M Duffy, Adam W Feinberg
Mar 20, 2014·Biotechnology and Bioengineering·Michael S GrigolaK Jimmy Hsia
Oct 17, 2015·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Naoya TakedaYusuke Hara
Dec 22, 2017·Journal of Tissue Engineering and Regenerative Medicine·Frank H KungBonnie L Firestein
Nov 26, 2015·Advanced Healthcare Materials·Yanfen Li, Kristopher A Kilian
Nov 20, 2016·Advanced Materials·Soumen JanaMiqin Zhang
Jan 1, 2017·Stem Cell Research & Therapy·Lekshmi KrishnaDebashish Das
Sep 28, 2017·Bioengineering·Jinah Jang
Apr 15, 2021·Advanced Biology·Che J Connon, Ricardo M Gouveia
Jan 23, 2014·Development·Brandi N Davis-DusenberyKevin Eggan
May 28, 2021·Journal of Biomedical Materials Research. Part a·Miranda M Carleton, Michael V Sefton
Aug 4, 2021·Essays in Biochemistry·Anita QuigleyRobert M I Kapsa
Mar 27, 2019·ACS Applied Materials & Interfaces·Clément RieuLéa Trichet

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