Convection-Enhanced Biopatterning with Recirculation of Hydrodynamically Confined Nanoliter Volumes of Reagents

Analytical Chemistry
Julien AutebertGovind V Kaigala

Abstract

We present a new methodology for efficient and high-quality patterning of biological reagents for surface-based biological assays. The method relies on hydrodynamically confined nanoliter volumes of reagents to interact with the substrate at the micrometer-length scale. We study the interplay between diffusion, advection, and surface chemistry and present the design of a noncontact scanning microfluidic device to efficiently present reagents on surfaces. By leveraging convective flows, recirculation, and mixing of a processing liquid, this device overcomes limitations of existing biopatterning approaches, such as passive diffusion of analytes, uncontrolled wetting, and drying artifacts. We demonstrate the deposition of analytes, showing a 2- to 5-fold increase in deposition rate together with a 10-fold reduction in analyte consumption while ensuring less than 6% variation in pattern homogeneity on a standard biological substrate. In addition, we demonstrate the recirculation of a processing liquid using a microfluidic probe (MFP) in the context of a surface assay for (i) probing 12 independent areas with a single microliter of processing liquid and (ii) processing a 2 mm(2) surface to create 170 antibody spots of 50 × 100 μm(2)...Continue Reading

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Citations

Sep 23, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·David P TaylorGovind V Kaigala
Oct 28, 2016·Chemical Communications : Chem Comm·J F CorsG V Kaigala
Jan 24, 2019·Chemical Society Reviews·Iago PereiroGovind V Kaigala
May 27, 2020·The Analyst·Rajamanickam Sivakumar, Nae Yoon Lee
Jul 10, 2019·Physical Review Letters·Federico ParatoreMoran Bercovici
Apr 10, 2019·Nature Biomedical Engineering·Aditya KashyapGovind V Kaigala
Aug 5, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Emmanuel DelamarcheGovind V Kaigala
Sep 28, 2021·Microsystems & Nanoengineering·Robert D LovchikGovind Kaigala

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