Convenient analysis of protein modification by chemical blotting with fluorogenic "click" reagents

Molecular BioSystems
Jun OhataZachary T Ball

Abstract

Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide-alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.

References

Nov 19, 2004·Organic Letters·Krishnamoorthy SivakumarQian Wang
Dec 7, 2007·Nature Biotechnology·Marta Fernández-SuárezAlice Y Ting
Aug 29, 2009·Angewandte Chemie·Ellen M Sletten, Carolyn R Bertozzi
Mar 24, 2010·Chemical Society Reviews·Céline Le DroumaguetQian Wang
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael BoyceCarolyn R Bertozzi
Jul 20, 2011·Accounts of Chemical Research·Marjoke F DebetsFloris L van Delft
Aug 6, 2011·Accounts of Chemical Research·John T Ngo, David A Tirrell
Oct 26, 2012·Journal of the American Chemical Society·Frédéric FriscourtGeert-Jan Boons
Nov 24, 2012·Chemical Communications : Chem Comm·Rituparna Kundu, Zachary T Ball
Jan 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Charng-Sheng TsaiChi-Huey Wong
Jan 21, 2014·ACS Chemical Biology·David M PattersonJennifer A Prescher
Jan 16, 2015·Current Opinion in Chemical Biology·Zachary T Ball
Sep 2, 2015·Chemical Communications : Chem Comm·Jun OhataZachary T Ball

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Citations

Dec 8, 2015·Expert Opinion on Biological Therapy·Chi-Jing ChoongHideki Mochizuki
Jun 2, 2016·Journal of the American Chemical Society·Jun OhataZachary T Ball
Dec 31, 2016·Bioconjugate Chemistry·Samuel C MartinZachary T Ball
Feb 9, 2018·Angewandte Chemie·Jun OhataZachary T Ball
Jan 23, 2018·Angewandte Chemie·Jun OhataZachary T Ball
Sep 21, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Jun Ohata, Zachary T Ball
Feb 16, 2019·Chemical Communications : Chem Comm·Kengo HanayaZachary T Ball
Sep 19, 2017·Chemical Communications : Chem Comm·Dan Wu, Donal F O'Shea
Mar 18, 2021·Organic & Biomolecular Chemistry·Yvette M C A LuijkxTom Wennekes
Aug 16, 2017·Journal of the American Chemical Society·Jun Ohata, Zachary T Ball

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