Convenient and effective method for removing fibrinogen from serum specimens before protein electrophoresis

Clinical Chemistry
Ling L QiuRonald J Elin

Abstract

Fibrinogen in serum specimens can be misinterpreted on protein electrophoresis as a monoclonal protein. We evaluated selective precipitation of fibrinogen with ethanol. Pooled human plasma was mixed with absolute ethanol or saline (final concentrations of 40, 80, 100, 120, and 160 mL/L) and incubated at 4 degrees C overnight or placed in an ice bath for 15 min. After centrifugation, the supernatants and resuspended pellets were used for protein electrophoresis and quantitative measurements of protein and fibrinogen. The fibrinogen band was effectively eliminated from the electrophoretic pattern in the plasma samples treated with ethanol at 100 mL/L and incubated in an ice bath for 15 min without a significant change in immunoglobulin concentrations. The 100 mL/L ethanol did not noticeably change the electrophoretic pattern of monoclonal immunoglobulins. This approach allowed analysis of a sample collected from an arteriovenous shunt kept open with heparin. Ethanol, 100 mL/L, can selectively precipitate fibrinogen without significantly interfering with the immunoglobulins. The precipitation process can be completed in 15 min at 0-4 degrees C and can avoid the need to obtain another blood sample.

Citations

Jan 28, 2012·American Journal of Veterinary Research·Benjamin W HaleJohn D Kisiday
Dec 6, 2008·Veterinary Clinical Pathology·Silvia RossiAlessia Giordano
Sep 16, 2011·Journal of Clinical Laboratory Analysis·Maria Teresa LeeBarbara Detrick
Sep 11, 2012·Veterinary Clinical Pathology·Giorgia ErricoSaverio Paltrinieri
Feb 10, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jennifer A SnyderDavid G Grenache
Dec 13, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yusheng Zhu, Ronald J Elin
May 19, 2007·Biochimica Et Biophysica Acta·Melvin E KlegermanDavid D McPherson
Mar 31, 2012·Clinical Chemistry·Jean-Baptiste OudartLaurent Ramont
Jan 26, 2021·Practical Laboratory Medicine·José María Gastélum-CanoMarcela Deffis-Court
Jun 15, 2021·Veterinary Dermatology·Devin FussellKeith E Linder

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