Conventional type 1 dendritic cells induce TH 1, TH 1-like follicular helper T cells and regulatory T cells after antigen boost via DEC205 receptor.

European Journal of Immunology
Fernando Bandeira SulczewskiSilvia Beatriz Boscardin

Abstract

Conventional dendritic cells (cDCs) are specialized in antigen presentation. In the mouse spleen, cDCs are classified in cDC1s and cDC2s, and express DEC205 and DCIR2 endocytic receptors, respectively. Monoclonal antibodies (mAbs) αDEC205 (αDEC) and αDCIR2 have been fused to different antigens to deliver them to cDC1s or cDC2s. We immunized mice with αDEC and αDCIR2 fused to an antigen using Poly(I:C) as adjuvant. The initial immune response was analyzed from days 3 to 6 after the immunization. We also studied the influence of a booster dose. Our results showed that antigen targeting to cDC1s promoted a pro-inflammatory TH 1 cell response. Antigen targeting to cDC2s induced TFH cells, GCs, and plasma cell differentiation. After boost, antigen targeting to cDC1s improved the TH 1 cell response and induced TH 1-like TFH cells that led to an increase in specific antibody titers and IgG class switch. Additionally, a population of regulatory T cells was also observed. Antigen targeting to cDC2s did not improve the specific antibody response after boost. Our results add new information on the immune response induced after the administration of a booster dose with αDEC and αDCIR2 fusion mAbs. These results may be useful for vaccine de...Continue Reading

References

Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M C NussenzweigB Gutchinov
Dec 9, 1998·Immunologic Research·J AlexanderA Sette
Sep 19, 2001·The Journal of Experimental Medicine·D HawigerM C Nussenzweig
May 22, 2002·The Journal of Experimental Medicine·Tomonori IyodaKayo Inaba
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Sep 17, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ralph M Steinman
Mar 17, 2004·The Journal of Experimental Medicine·Laura C BonifazRalph M Steinman
Mar 1, 2006·The Journal of Experimental Medicine·Silvia B BoscardinMichel C Nussenzweig
Mar 1, 2006·The Journal of Experimental Medicine·Christine TrumpfhellerRalph M Steinman
Jan 6, 2007·Science·Diana DudziakMichel C Nussenzweig
Nov 1, 2007·European Journal of Immunology·Ralph M Steinman
Feb 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christine TrumpfhellerRalph M Steinman
Mar 8, 2008·The Journal of Clinical Investigation·Godwin NchindaRalph M Steinman
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sayuri YamazakiRalph M Steinman
Jun 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isharat YusufShane Crotty
Feb 15, 2011·Annual Review of Immunology·Shane Crotty
Jun 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mireille H LahoudIrina Caminschi
Sep 21, 2011·The Journal of Experimental Medicine·Mercedes B FuertesThomas F Gajewski
Dec 27, 2011·Immunity·Shingo NakayamadaJohn J O'Shea
Jul 4, 2012·The Journal of Experimental Medicine·Cindy S MaStuart G Tangye
Sep 12, 2012·The Journal of Experimental Medicine·Craig P ChappellEdward A Clark
Mar 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Youn Soo ChoiShane Crotty
May 16, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kirsten NeubertDiana Dudziak
Jul 19, 2014·Nature Reviews. Immunology·Martin GuilliamsSimon Yona
Aug 23, 2016·Cell Reports·Samuele CalabroStephanie C Eisenbarth
Feb 23, 2017·Frontiers in Immunology·Juliana de Souza ApostólicoDaniela Santoro Rosa

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