Convergence of cAMP and phosphoinositide pathways during rat parotid secretion.

The American Journal of Physiology
J S McKinneyR P Rubin

Abstract

Rat parotid acinar cells were employed to investigate the mechanism by which receptor agonists that activate the phosphoinositide pathway enhance the stimulatory effects of adenosine 3',5'-cyclic monophosphate (cAMP) on amylase secretion. Norepinephrine (NE), which activates both alpha- and beta-adrenoceptors, evoked a secretory response that was greater than the sum of the responses obtained when NE was employed as a beta-agonist (in the presence of prazosin) and as an alpha-agonist (in the presence of propranolol). The enhancement of amylase secretion induced by NE was accompanied by an augmented rise in Ca2+ influx, as determined by fura-2 analysis. NE-induced cAMP production was comparable to that evoked by NE as a beta-agonist, and the accumulation of [3H]inositol 1,4,5-trisphosphate (IP3) evoked by NE was comparable to that elicited by NE as an alpha-agonist. The beta-adrenoceptor agonist isoproterenol potentiated the rise in cytosolic Ca2+ elicited by the muscarinic agonist carbachol, while possessing no stimulatory effect of its own. Isoproterenol had no effect on carbachol-induced stimulation of [3H]IP3 or 1,3,4,5-[3H]inositol tetrakisphosphate accumulation. Ionomycin and dibutyryl cAMP in combination produced a simila...Continue Reading

References

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Citations

May 1, 1993·Journal of Cellular Physiology·D P Chopra, I C Xue-Hu
Feb 3, 1999·Annals of the New York Academy of Sciences·E KabréJ P Dehaye
Aug 4, 1998·The American Journal of Physiology·Y ZhuR P Rubin
Oct 9, 2002·The Journal of Biological Chemistry·Jason I E BruceTrevor J Shuttleworth
Jan 1, 1995·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·K R Purushotham, M G Humphreys-Beher
Oct 31, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Dagnia LoomsSteen Dissing
Jun 4, 1998·Biochemical and Biophysical Research Communications·T Ozawa
Jul 9, 1999·Biochimica Et Biophysica Acta·R D RaffanielloG K Ojakian
Sep 29, 2004·Biochemical Pharmacology·Lucila BuschEnri Borda

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