Convergence of virulence and MDR in a single plasmid vector in MDR Klebsiella pneumoniae ST15.

The Journal of Antimicrobial Chemotherapy
Margaret M C LamIren H Lohr

Abstract

MDR and hypervirulence (hv) are typically observed in separate Klebsiella pneumoniae populations. However, convergent strains with both properties have been documented and potentially pose a high risk to public health in the form of invasive infections with limited treatment options. Our aim was to characterize the genetic determinants of virulence and antimicrobial resistance (AMR) in two ESBL-producing K. pneumoniae isolates belonging to the international MDR clone ST15. The complete genome sequences of both isolates, including their plasmids, were resolved using Illumina and Oxford Nanopore sequencing. Both isolates carried large mosaic plasmids in which AMR and virulence loci have converged within the same vector. These closely related mosaic hv-MDR plasmids include sequences typical of the K. pneumoniae virulence plasmid 1 (KpVP-1; including aerobactin synthesis locus iuc) fused with sequences typical of IncFIIK conjugative AMR plasmids. One hv-MDR plasmid carried three MDR elements encoding the ESBL gene blaCTX-M-15 and seven other AMR genes (blaTEM, aac3'-IIa, dfrA1, satA2, blaSHV, sul1 and aadA1). The other carried remnants of these elements encoding blaTEM and aac3'-IIa, and blaCTX-M-15 was located in a second plasmid ...Continue Reading

References

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Citations

Nov 4, 2019·Journal of Internal Medicine·J E ChobyD S Weiss
Apr 7, 2020·Nature Ecology & Evolution·Hannah JordtBenjamin Kerr
Sep 24, 2020·MBio·Kimberly A WalkerVirginia L Miller
Feb 15, 2020·Nature Reviews. Microbiology·Kelly L WyresKathryn E Holt
Jul 2, 2019·Emerging Microbes & Infections·Nadia Rodríguez-MedinaUlises Garza-Ramos
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Chaitra ShankarBalaji Veeraraghavan
Feb 10, 2021·Infection and Immunity·Shekina Gonzalez-FerrerDaria Van Tyne

Datasets Mentioned

BETA
SAMEA5063299
SAMEA5063300
CP034046
CP034054
CP018313.1
AP000342.1
ERR1415588

Methods Mentioned

BETA
ICE

Related Concepts

Whole Genome Sequencing
Bacteriocidal Agents
Homo sapiens
Klebsiella Infections
Klebsiella rhinoscleromatis
Breakpoint Determination, Antibacterial Susceptibility
Norway
Phylogeny
Episomes
Pathogenicity

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