Convergence of virulence and MDR in a single plasmid vector in MDR Klebsiella pneumoniae ST15.

The Journal of Antimicrobial Chemotherapy
Margaret M C LamIren H Lohr


MDR and hypervirulence (hv) are typically observed in separate Klebsiella pneumoniae populations. However, convergent strains with both properties have been documented and potentially pose a high risk to public health in the form of invasive infections with limited treatment options. Our aim was to characterize the genetic determinants of virulence and antimicrobial resistance (AMR) in two ESBL-producing K. pneumoniae isolates belonging to the international MDR clone ST15. The complete genome sequences of both isolates, including their plasmids, were resolved using Illumina and Oxford Nanopore sequencing. Both isolates carried large mosaic plasmids in which AMR and virulence loci have converged within the same vector. These closely related mosaic hv-MDR plasmids include sequences typical of the K. pneumoniae virulence plasmid 1 (KpVP-1; including aerobactin synthesis locus iuc) fused with sequences typical of IncFIIK conjugative AMR plasmids. One hv-MDR plasmid carried three MDR elements encoding the ESBL gene blaCTX-M-15 and seven other AMR genes (blaTEM, aac3'-IIa, dfrA1, satA2, blaSHV, sul1 and aadA1). The other carried remnants of these elements encoding blaTEM and aac3'-IIa, and blaCTX-M-15 was located in a second plasmid ...Continue Reading


Mar 25, 2009·Antimicrobial Agents and Chemotherapy·Alessandra Carattoli
May 25, 2011·International Journal of Antimicrobial Agents·Mi Young LeeJae-Hoon Song
Mar 7, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S BreurecSylvain Brisse
Jun 19, 2012·Future Microbiology·Alyssa S Shon, Thomas A Russo
Mar 20, 2014·Bioinformatics·Torsten Seemann
Nov 22, 2014·Nucleic Acids Research·Nicholas J CroucherSimon R Harris
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kathryn E HoltNicholas R Thomson
Oct 16, 2016·Trends in Microbiology·Kelly L Wyres, Kathryn E Holt
Nov 22, 2016·The Lancet Infectious Diseases·Hajo GrundmannEuropean Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group
Jun 9, 2017·PLoS Computational Biology·Ryan R WickKathryn E Holt
Sep 3, 2017·The Lancet Infectious Diseases·Liang Chen, Barry N Kreiswirth
Nov 6, 2017·The Lancet Infectious Diseases·Marcus Ho Yin WongSheng Chen
Nov 6, 2017·The Lancet Infectious Diseases·Hong YaoXiang-Dang Du


Nov 4, 2019·Journal of Internal Medicine·J E ChobyD S Weiss
Apr 7, 2020·Nature Ecology & Evolution·Hannah JordtBenjamin Kerr
Sep 24, 2020·MBio·Kimberly A WalkerVirginia L Miller
Feb 15, 2020·Nature Reviews. Microbiology·Kelly L WyresKathryn E Holt
Jul 2, 2019·Emerging Microbes & Infections·Nadia Rodríguez-MedinaUlises Garza-Ramos
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Chaitra ShankarBalaji Veeraraghavan
Feb 10, 2021·Infection and Immunity·Shekina Gonzalez-FerrerDaria Van Tyne

Datasets Mentioned


Methods Mentioned


Related Concepts

Whole Genome Sequencing
Bacteriocidal Agents
Homo sapiens
Klebsiella Infections
Klebsiella rhinoscleromatis
Breakpoint Determination, Antibacterial Susceptibility

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.