Convergent gain and loss of genomic islands drive lifestyle changes in plant-associated Pseudomonas.

The ISME Journal
Ryan A MelnykCara H Haney

Abstract

Host-associated bacteria can have both beneficial and detrimental effects on host health. While some of the molecular mechanisms that determine these outcomes are known, little is known about the evolutionary histories of pathogenic or mutualistic lifestyles. Using the model plant Arabidopsis, we found that closely related strains within the Pseudomonas fluorescens species complex promote plant growth and occasionally cause disease. To elucidate the genetic basis of the transition between commensalism and pathogenesis, we developed a computational pipeline and identified genomic islands that correlate with outcomes for plant health. One island containing genes for lipopeptide biosynthesis and quorum-sensing is required for pathogenesis. Conservation of the quorum-sensing machinery in this island allows pathogenic strains to eavesdrop on quorum signals in the environment and coordinate pathogenic behavior. We found that genomic loci associated with both pathogenic and commensal lifestyles were convergently gained and lost in multiple lineages through homologous recombination, possibly constituting an early step in the differentiation of pathogenic and commensal lifestyles. Collectively this work provides novel insights into the ...Continue Reading

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Citations

Dec 24, 2018·Applied and Environmental Microbiology·Mark J MandelKaren Guillemin
Aug 23, 2019·Natural Product Reports·Sebastian Götze, Pierre Stallforth
Sep 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tyler C HelmannSteven E Lindow
Sep 5, 2020·Critical Reviews in Microbiology·Léa GirardRené De Mot
Jun 13, 2020·Annual Review of Microbiology·Connor R FitzpatrickJeffery L Dangl
Oct 17, 2019·Frontiers in Microbiology·Marc G ChevretteCameron R Currie
Feb 6, 2021·Current Opinion in Plant Biology·Siyu SongCara H Haney
Sep 29, 2019·Current Opinion in Microbiology·Paulo José Pl TeixeiraJeffery L Dangl
Apr 21, 2021·Nature Reviews. Microbiology·Georgia C DrewKayla C King
Apr 15, 2020·Cell Host & Microbe·Kelsey E HuusB Brett Finlay

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Datasets Mentioned

BETA
GCA001023535

Methods Mentioned

BETA
biosensor
PCR
amplicon-based sequencing

Software Mentioned

USEARCH
OrthoFinder2
PyParanoid
ImageJ
treeWAS

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