Converse modulation of IRP1 and IRP2 by immunological stimuli in murine RAW 264.7 macrophages.

The Journal of Biological Chemistry
Cécile BoutonJean-Claude Drapier

Abstract

Iron regulatory proteins (IRP1 and IRP2) are two cytoplasmic RNA-binding proteins that control iron metabolism in mammalian cells. Both IRPs bind to specific sequences called iron-responsive elements (IREs) located in the 3' or 5' untranslated regions of several mRNAs, in particular mRNA encoding ferritin and transferrin receptor. In this study, we followed in parallel the in vivo regulation of the two IRPs in physiologically stimulated macrophages. We show that stimulation of mouse RAW 264.7 macrophage-like cells increased IRP1 IRE binding activity 4-fold, whereas IRP2 activity decreased 2-fold 8 h after interferon-gamma/lipopolysaccharide treatment. Decrease in IRP2 was not due to nitric oxide (NO) production and did not require de novo protein synthesis. Our data therefore indicate that the two IRPs can be conversely regulated in response to the same stimulus. In addition, the effect of endogenously produced NO on IRP1 was further characterized in an activated macrophage/target cell system. We show that NO acts as an intercellular signal to increase IRP1 activity in adjacent cells. As the effect was detectable within 1 h and did not require de novo protein synthesis, this result supports a direct action of NO on IRP1.

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M C KennedyH Beinert
Dec 1, 1992·Archives of Biochemistry and Biophysics·L ZhengH Zalkin
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J HaileR D Klausner
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E A Leibold, H N Munro
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J L CaseyJ B Harford
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N Aziz, H N Munro
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·K Pantopoulos, M W Hentze
Sep 15, 1995·The Journal of Biological Chemistry·B GuoE A Leibold
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R JaffreyS H Snyder
Nov 15, 1994·The Biochemical Journal·E C Theil
Dec 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H Beinert, M C Kennedy
Mar 1, 1996·The Journal of Biological Chemistry·B R HendersonL C Kühn
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·J ButtT A Rouault
Mar 22, 1996·The Journal of Biological Chemistry·K L Schalinske, R S Eisenstein
Jul 1, 1996·Molecular and Cellular Biology·K PantopoulosM W Hentze
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M W Hentze, L C Kühn
Jan 1, 1996·Methods in Enzymology·J C Drapier, J B Hibbs
Aug 8, 1997·The Journal of Biological Chemistry·C BoutonJ C Drapier
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·K L SchalinskeR S Eisenstein

❮ Previous
Next ❯

Citations

Sep 29, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Chidi V NwenekaAndrew Prentice
Feb 24, 2001·Molecular Aspects of Medicine·P T LieuY Yang
Aug 2, 2001·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·H TapieroK D Tew
Jan 11, 2000·The Proceedings of the Nutrition Society·J H Brock, V Mulero
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Douglas T HessJonathan S Stamler
Sep 8, 1999·The Biochemical Journal·Y Morel, R Barouki
Nov 22, 2000·The Biochemical Journal·G Cairo, A Pietrangelo
Jun 6, 2002·The Plant Journal : for Cell and Molecular Biology·Irene MurgiaCarlo Soave
Feb 3, 2005·Molecular and Cellular Biology·Jian WangKostas Pantopoulos
Aug 15, 2000·Annual Review of Nutrition·R S Eisenstein
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Oliveira, J C Drapier
May 9, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ruth DanzeisenMaria Teresa Carrì
Feb 18, 2006·Free Radical Research·Premysl MladenkaRadomír Hrdina
Feb 24, 2009·Journal of Inorganic Biochemistry·Cédric FossetHarry J McArdle
Sep 13, 2008·Biochimica Et Biophysica Acta·Günter Weiss
Apr 6, 2004·Journal of Clinical Laboratory Analysis·Roger S RileyGina Romagnoli
Dec 30, 2009·European Journal of Immunology·Stefania RecalcatiGaetano Cairo
Apr 6, 2002·Clinics in Laboratory Medicine·Roger S RileyJohn Cousar
May 1, 2002·Redox Report : Communications in Free Radical Research·Carine Fillebeen, Kostas Pantopoulos
Apr 21, 2007·Acta Physiologica Hungarica·J BallaGy Balla
Oct 14, 2014·Metallomics : Integrated Biometal Science·Lukas C Kühn
May 15, 2003·Science's STKE : Signal Transduction Knowledge Environment·Cécile Bouton, Jean-Claude Drapier
Oct 3, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Dec 9, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Jul 30, 2004·American Journal of Physiology. Cell Physiology·Jau-Yi LiJonathan Barasch
Dec 30, 1998·The Journal of Nutrition·R S Eisenstein, K P Blemings
Jan 10, 2021·Antioxidants·Pamela J UrrutiaMarco Tulio Núñez
Jan 28, 2003·Blood Cells, Molecules & Diseases·Sangwon Kim, Prem Ponka

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.