Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440

Metabolic Engineering Communications
Michael T GuarnieriGregg T Beckham

Abstract

The sugar dehydration products, furfural and 5-(hydroxymethyl)furfural (HMF), are commonly formed during high-temperature processing of lignocellulose, most often in thermochemical pretreatment, liquefaction, or pyrolysis. Typically, these two aldehydes are considered major inhibitors in microbial conversion processes. Many microbes can convert these compounds to their less toxic, dead-end alcohol counterparts, furfuryl alcohol and 5-(hydroxymethyl)furfuryl alcohol. Recently, the genes responsible for aerobic catabolism of furfural and HMF were discovered in Cupriavidus basilensis HMF14 to enable complete conversion of these compounds to the TCA cycle intermediate, 2-oxo-glutarate. In this work, we engineer the robust soil microbe, Pseudomonas putida KT2440, to utilize furfural and HMF as sole carbon and energy sources via complete genomic integration of the 12 kB hmf gene cluster previously reported from Burkholderia phytofirmans. The common intermediate, 2-furoic acid, is shown to be a bottleneck for both furfural and HMF metabolism. When cultured on biomass hydrolysate containing representative amounts of furfural and HMF from dilute-acid pretreatment, the engineered strain outperforms the wild type microbe in terms of reduc...Continue Reading

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Citations

Jul 11, 2019·International Journal of Molecular Sciences·Ying WangShao-Yuan Leu
Aug 14, 2020·Applied Microbiology and Biotechnology·Anna WeimerChristoph Wittmann
Dec 11, 2019·Applied Microbiology and Biotechnology·Haibo YuanLong Liu
Jan 1, 2020·Biotechnology for Biofuels·George L PeabodyAdam M Guss
Jun 5, 2020·Current Opinion in Biotechnology·Anita Loeschcke, Stephan Thies
Apr 14, 2020·Metabolic Engineering Communications·Matthew R InchaJay D Keasling
Apr 19, 2021·Metabolic Engineering·Till TisoLars M Blank
May 18, 2021·Computational and Structural Biotechnology Journal·Raúl A DonosoDanilo Pérez-Pantoja
Nov 11, 2021·Journal of Agricultural and Food Chemistry·Qingxuan MuBo Yu

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