PMID: 11919549Mar 29, 2002Paper

Conversion of 7 alpha-hydroxycholesterol to bile acid in human subjects: is there an alternate pathway favoring cholic acid synthesis?

The Journal of Laboratory and Clinical Medicine
W C Duane, N B Javitt

Abstract

Despite the fact that most human subjects synthesize about twice as much cholic acid as chenodeoxycholic acid, available evidence suggests that 7 alpha-hydroxycholesterol, the first intermediate in the major pathway for bile acid synthesis, is converted about equally to these two bile acids. Synthesis through the main alternate pathway can not explain this discrepancy because 27-hydroxycholesterol, the first intermediate in that pathway, is converted preferentially to chenodeoxycholic acid. To examine the validity of these contradictory observations, we administered (24-(14)C)-cholic acid and (24-(14)C)-chenodeoxycholic acid together with (7 beta-(3)H)-7 alpha-hydroxycholesterol on one occasion and (22,23-(3)H)-27-hydroxycholesterol on a separate occasion to eight normal human subjects. Synthesis of the two primary bile acids was determined by means of standard isotope dilution kinetics of the carbon 14-specific activities of biliary bile acids. Conversion of (7 beta-(3)H)-7 alpha-hydroxycholesterol and (22,23-(3)H)-27-hydroxycholesterol to bile acid was calculated from the tritium/carbon 14 ratio in cholic and chenodeoxycholic acid. For synthesis, the mean +/- SEM cholic/chenodeoxycholic ratio was 1.82 +/- 0.26. For apparent c...Continue Reading

References

Jul 1, 1988·The Journal of Clinical Investigation·W C DuaneJ N Hamilton
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May 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M AxelsonC Einarsson

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Citations

Oct 12, 2010·Tissue Engineering. Part a·Christopher J DetzelPadmavathy Rajagopalan
Mar 13, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael Fuchs
Feb 22, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marco BertolottiMarina Del Puppo
Apr 12, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Marina Del PuppoMarzia Galli Kienle
Jan 3, 2018·The Journal of Organic Chemistry·Desta Doro BumeThomas Lectka

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