Conversion of a ribozyme to a deoxyribozyme through in vitro evolution

Chemistry & Biology
Natasha PaulGerald F Joyce

Abstract

An RNA ligase ribozyme was converted to a corresponding deoxyribozyme through in vitro evolution. The ribozyme was prepared as a DNA molecule of the same sequence, and had no detectable activity. A population of randomized variants of this DNA was constructed and evolved to perform RNA ligation at a rate similar to that of the starting ribozyme. When the deoxyribozyme was prepared as an RNA molecule of the same sequence, it had no detectable activity. Thus, the evolutionary transition from an RNA to a DNA enzyme represents a switch, rather than a broadening, of the chemical basis for catalytic function. This transfer of both information and function is relevant to the transition between two different genetic systems based on nucleic acid-like molecules, as postulated to have occurred during the early history of life on Earth.

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Citations

May 4, 2012·Journal of the American Chemical Society·Charles OleaGerald F Joyce
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Simon G TrevinoJack W Szostak
Dec 23, 2008·Antioxidants & Redox Signaling·Gloria Saab-Rincón, Brenda Valderrama
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Mar 26, 2009·Chemistry & Biology·Kenny Schlosser, Yingfu Li
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Nov 5, 2014·Nature·Jonathan T Sczepanski, Gerald F Joyce
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Jan 3, 2019·Annual Review of Biophysics·Celia BlancoIrene A Chen
Jul 25, 2014·Chembiochem : a European Journal of Chemical Biology·Chih-Chi ChuScott K Silverman
Dec 14, 2017·Quarterly Reviews of Biophysics·Falk WachowiusPhilipp Holliger
May 25, 2021·Journal of the American Chemical Society·Yao WangHanyang Yu
Feb 15, 2008·Journal of the American Chemical Society·Madhavaiah Chandra, Scott K Silverman

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