Conversion of Broad-Spectrum Antimicrobial Peptides into Species-Specific Antimicrobials Capable of Precisely Targeting Pathogenic Bacteria.

Scientific Reports
Lin XuNa Dong

Abstract

Currently, the majority of antibiotics in clinical use have broad activity spectra, killing pathogenic and beneficial microorganisms indiscriminately. The disruption of the ecological balance of normal flora often results in secondary infections or other antibiotic-associated complications. Therefore, targeted antimicrobial therapies capable of specifically eliminating pathogenic bacteria while retaining the protective benefits of a normal microflora would be advantageous. In this study, we successfully constructed a series of Enterococcus faecalis-targeted antimicrobial peptides from wide-spectrum antimicrobial peptide precursors. These peptides are designed based on fusion of the species-specific peptide pheromone cCF10 and modification of the active region of the antimicrobial peptide. The results showed that cCF10-C4 possessed specific antimicrobial activity against E. faecalis and was not active against other types of bacteria tested. The specificity of this hybrid peptide was shown by the absence of antimicrobial effects in the pheromone-substituted derivative. Further studies indicated that cCF10-C4 and its parent peptide C4 exert their activities by damaging cytoplasmic membrane integrity. The present study reveals the ...Continue Reading

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Citations

Jun 13, 2020·Frontiers in Immunology·Darren Shu Jeng TingImran Mohammed
Dec 19, 2020·International Journal of Molecular Sciences·Stanislav R KurpeOxana V Galzitskaya
Nov 17, 2020·Frontiers in Microbiology·Rojita MishraJunaid Khan
Jan 11, 2021·European Journal of Medicinal Chemistry·Chao ZhongJingman Ni
Feb 23, 2021·ACS Pharmacology & Translational Science·Bhuvaneshwari BalasubramaniamRaju Kumar Gupta
Apr 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nuno Mariz-PonteCátia Teixeira
Oct 15, 2020·Applied Microbiology and Biotechnology·Paula Roszczenko-JasińskaElżbieta K Jagusztyn-Krynicka
Feb 20, 2021·Canadian Journal of Microbiology·Celine Stoica, Georgina Cox

Methods Mentioned

BETA
electron microscopy
two hybrid
flow cytometry
Fluorescence

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