Conversion of cadherin isoforms in cultured human gastric carcinoma cells

World Journal of Gastroenterology : WJG
Bing-Jing WangYang Ke

Abstract

To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation. The expressions of cell adhesion molecules (including E-cadherin, N-cadherin, alpha-catenin, beta-catenin) and cadherin transcription factors including snail, slug and twist were determined by reverse transcriptase-polymerase chain reaction(RT-PCR), immunoblotting and immunofluorescence in SV40-immortalized human gastric cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901. All cell lines expressed N-cadherin, but not E-cadherin. N-cadherin immunofluorescence was detected at cell membranous adherents junctions where co-localization with immunofluorescent staining of inner surface adhesion proteins alpha- and beta-catenins was observed. The transformed Ges-1 and gastric cancer cell lines all expressed transcription factors (snail, slug and twist) which inhibited the expression of E-cadherin and triggered epithelial-mesenchymal transformation. Cadherin isoforms can change from E-cadherin to N-cadherin in transformed human gastric cancer cells, which is associated with intracellular events of stomach carcinogenesis and high expression of corresponding transcription factors.

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Citations

Sep 17, 2013·International Journal of Cancer. Journal International Du Cancer·Shiwei GuoYajun Guo
Nov 27, 2019·Cell Communication and Signaling : CCS·Patrícia CarneiroRaquel Seruca
Aug 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Natalia YanchenkoTakanori Hattori
Mar 25, 2017·American Journal of Reproductive Immunology : AJRI·Jin Hee AhnJoanne Kwak-Kim
Aug 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Birgit HotzHubert G Hotz

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