Conversion of DNA gyrase into a conventional type II topoisomerase

Proceedings of the National Academy of Sciences of the United States of America
S C Kampranis, A Maxwell

Abstract

DNA gyrase is unique among topoisomerases in its ability to introduce negative supercoils into closed-circular DNA. We have demonstrated that deletion of the C-terminal DNA-binding domain of the A subunit of gyrase gives rise to an enzyme that cannot supercoil DNA but relaxes DNA in an ATP-dependent manner. Novobiocin, a competitive inhibitor of ATP binding by gyrase, inhibits this reaction. The truncated enzyme, unlike gyrase, does not introduce a right-handed wrap when bound to DNA and stabilizes DNA crossovers; characteristics reminiscent of conventional type II topoisomerases. This new enzyme form can decatenate DNA circles with increased efficiency compared with intact gyrase and, as a result, can complement the temperature-sensitive phenotype of a parCts mutant. Thus these results suggest that the unique properties of DNA gyrase are attributable to the wrapping of DNA around the C-terminal DNA-binding domains of the A subunits and provide an insight into the mechanism of type II topoisomerases.

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertH A Nash
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A SuginoN R Cozzarelli
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M GellertM H O'Dea
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, J C Wang
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M GellertJ I Tomizawa
Oct 1, 1992·The Journal of Antimicrobial Chemotherapy·A Maxwell
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·R J Reece, A Maxwell
Jan 1, 1988·Cell Biophysics·H V WesterhoffM Gellert
Jan 1, 1989·Annual Review of Biochemistry·L F Liu
Mar 31, 1988·Biochimica Et Biophysica Acta·S Tornaletti, A M Pedrini
May 1, 1980·Cell·K N Kreuzer, N R Cozzarelli
Nov 11, 1982·Nucleic Acids Research·N P Higgins, N R Cozzarelli
Oct 20, 1995·The Journal of Biological Chemistry·H Peng, K J Marians
Nov 1, 1994·The Biochemical Journal·P M Watt, I D Hickson
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H Peng, K J Marians
Jan 18, 1996·Nature·J M BergerJ C Wang
Jan 1, 1996·Annual Review of Biochemistry·J C Wang
Feb 28, 1996·Inorganic Chemistry·Zuolin ZhuJames H. Espenson

❮ Previous
Next ❯

Citations

Mar 17, 2004·Journal of Molecular Biology·Jonathan G HeddleNeil H Thomson
Jun 8, 2002·Journal of Molecular Biology·Susan C Hockings, Anthony Maxwell
Sep 28, 1998·Biochimica Et Biophysica Acta·J M Berger
Jul 20, 2002·Current Opinion in Structural Biology·Ruth M Saecker, M Thomas Record
Mar 31, 1998·Current Opinion in Structural Biology·J M Berger
Aug 26, 1998·Trends in Microbiology·M CouturierL Van Melderen
Aug 30, 2008·Quarterly Reviews of Biophysics·Allyn J Schoeffler, James M Berger
Dec 10, 2013·Chemical Reviews·Claudine Mayer, Yves L Janin
Jan 7, 2006·Nature·Jeff GoreCarlos Bustamante
Nov 24, 2011·Nature Reviews. Molecular Cell Biology·Seychelle M VosJames M Berger
Apr 10, 2012·Nature Structural & Molecular Biology·Aakash BasuZev Bryant
Mar 6, 2007·Nature Structural & Molecular Biology·Marcelo NöllmannNicholas R Cozzarelli
Nov 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Elsa M TretterJames M Berger
Dec 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Keir C Neuman
Oct 19, 2010·The Journal of Biological Chemistry·Jie YuanMatthew K Waldor
Mar 29, 2001·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·E Fernandez-MoreiraA G de la Campa
Jul 15, 1997·Nucleic Acids Research·M E CoveA Maxwell
Sep 28, 2010·Nucleic Acids Research·Nicole M BakerAlfonso Mondragón
Jul 31, 2012·Nucleic Acids Research·Shantanu KarkareAnthony Maxwell
Sep 20, 2012·Nucleic Acids Research·Rupesh KumarValakunja Nagaraja
Sep 15, 2012·Nucleic Acids Research·Martin A Lanz, Dagmar Klostermeier
Mar 29, 2001·Genes & Development·R W DeiblerE L Zechiedrich
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·John H TranDavid C Hooper
May 31, 2012·Molekuliarnaia biologiia·D A Giliarov, I S Shkundina
Jun 8, 2001·Annual Review of Biochemistry·J J Champoux
Apr 28, 2012·BMC Complementary and Alternative Medicine·Pinanong Na PhatthalungSupayang P Voravuthikunchai
Jul 17, 2010·Algorithms for Molecular Biology : AMB·Pavel V MazinOlga V Kalinina
Oct 22, 2013·PloS One·Klaus O Kopec, Andrei N Lupas
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·S C KampranisA Maxwell
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·O GuipaudP Forterre
Mar 19, 2013·Annual Review of Biochemistry·Stefanie Hartman ChenTao-shih Hsieh
Feb 15, 2001·Current Infectious Disease Reports·Darrin J. Bast, Joyce C. S. de Azavedo
Feb 19, 2010·Cellular and Molecular Life Sciences : CMLS·Claudia Sissi, Manlio Palumbo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Kevin D CorbettJames M Berger
Proceedings of the National Academy of Sciences of the United States of America
S C KampranisA Maxwell
Journal of Molecular Biology
Jonathan G HeddleNeil H Thomson
© 2021 Meta ULC. All rights reserved