Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators

Scientific Reports
Shu WeiLiangxue Lai

Abstract

The recently emerged CRISPR/Cas9 technique has opened a new perspective on readily editing specific genes. When combined with transcription activators, it can precisely manipulate endogenous gene expression. Here, we enhanced the expression of endogenous Cdx2 and Gata6 genes by CRISPR-mediated activators, thus mouse embryonic stem cells (ESCs) were directly converted into two extraembryonic lineages, i.e., typical trophoblast stem cells (TSCs) and extraembryonic endoderm cells (XENCs), which exhibited characters of TSC or XENC derived from the blastocyst extraembryonic lineages such as cell morphology, specific gene expression, and differentiation ability in vitro and in vivo. This study demonstrates that the cell fate can be effectively manipulated by directly activating of specific endogenous gene expression with CRISPR-mediated activator.

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Citations

May 31, 2017·Cellular Reprogramming·Kai XiongYonglun Luo
Jun 22, 2017·Annual Review of Biomedical Engineering·Joshua B BlackCharles A Gersbach
Nov 7, 2019·Journal of Cellular Physiology·Navid ShomaliFaroogh Marofi
Oct 27, 2016·Chembiochem : a European Journal of Chemical Biology·Matthew D WiensRobert E Campbell
Jul 8, 2018·Nature Communications·Jere WeltnerTimo Otonkoski
Jan 7, 2021·International Journal of Molecular Sciences·Nasir JavaidSangdun Choi
May 12, 2021·Trends in Biotechnology·Jacob H Goell, Isaac B Hilton
Jun 10, 2020·Current Gene Therapy·Mahmoud GharbaviSaeed Ghanbarzadeh

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Methods Mentioned

BETA
PCR
transfection
flow cytometry

Software Mentioned

GraphPad Prism
FlowJo

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