PMID: 9440535Jan 24, 1998Paper

Conversion of NfsA, the major Escherichia coli nitroreductase, to a flavin reductase with an activity similar to that of Frp, a flavin reductase in Vibrio harveyi, by a single amino acid substitution

Journal of Bacteriology
S ZennoK Saigo

Abstract

NfsA is the major oxygen-insensitive nitroreductase of Escherichia coli, similar in amino acid sequence to Frp, a flavin reductase of Vibrio harveyi. Here, we show that a single amino acid substitution at position 99, which may destroy three hydrogen bonds in the putative active center, transforms NfsA from a nitroreductase into a flavin reductase that is as active as the authentic Frp and a tartrazine reductase that is 30-fold more active than wild-type NfsA.

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Citations

Jul 14, 2000·Journal of Bacteriology·M V Fonseca, J C Escalante-Semerena
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·A Esteve-NúñezJ L Ramos
Jan 5, 2002·Antioxidants & Redox Signaling·S C Tu
Jan 14, 2009·The Journal of Biological Chemistry·Zachary T Campbell, Thomas O Baldwin
Sep 24, 1999·Protein Science : a Publication of the Protein Society·J J TannerK L Krause
Mar 28, 2002·Applied and Environmental Microbiology·R Guy Riefler, Barth F Smets
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·E DíazJ L García
Jan 26, 2008·FEMS Microbiology Letters·Diederik Johannes Opperman, Esta van Heerden
Sep 4, 2001·Biotechnology & Genetic Engineering Reviews·C E FrenchN C Bruce
Mar 22, 2008·FEMS Microbiology Reviews·María Dolores RoldánConrado Moreno-Vivián
Apr 20, 2005·Applied Microbiology and Biotechnology·Hong YinBarth F Smets
Oct 9, 1999·Journal of Bacteriology·D S BlehertG H Chambliss
Aug 27, 2018·Journal of Colloid and Interface Science·Felipe BrunaVanessa Prevot
Aug 8, 2001·Archives of Biochemistry and Biophysics·M A JorgensenG L Mendz
Aug 28, 2021·International Journal of Molecular Sciences·Narimantas ČėnasLidija Kosychova
Nov 27, 2002·Biochemistry·Ronald L KoderAnne-Frances Miller

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