PMID: 6405382Feb 1, 1983Paper

Conversion of prohistidine decarboxylase to histidine decarboxylase: peptide chain cleavage by nonhydrolytic serinolysis

Proceedings of the National Academy of Sciences of the United States of America
P A RecseiE E Snell

Abstract

Unlabeled prohistidine decarboxylase and prohistidine decarboxylase containing L-[carboxyl-(18)O]serine or L-[hydroxyl-(18)O]serine were isolated in homogeneous form from mutant 3 of Lactobacillus 30a grown with the appropriately labeled serine. There was no randomization or redistribution of label during growth, isolation of the protein, or enzymatic hydrolysis and reisolation of the labeled amino acids. These proteins were used to show that during proenzyme activation, in which individual pi subunits of the proenzyme are converted to alpha and beta subunits of the active enzyme [Formula: see text] (in which pi, alpha, and beta subunits have the partial structures shown and Prv designates a pyruvoyl group), no (18)O from H(2) (18)O is incorporated into the newly formed carboxyl terminus (Ser-81) of the beta chain, although no labilization of (18)O from proenzyme labeled with L-[carboxyl-(18)O]serine occurred when the proenzyme was activated in H(2) (16)O by the same procedures. The additional oxygen atom present in the carboxyl group of Ser-81 of the beta subunit is transferred from the hydroxyl group of Ser-82 of the proenzyme during the activation reaction. The same result was obtained with wild-type enzyme formed intracellu...Continue Reading

Citations

Jun 8, 2001·Progress in Biophysics and Molecular Biology·P Chakrabarti, D Pal
Jul 21, 1999·Molecular and Biochemical Parasitology·L N KinchM A Phillips
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P D van Poelje, E E Snell
Jul 29, 2011·Critical Reviews in Food Science and Nutrition·Daniel M LinaresMaría Fernández
Apr 1, 2010·Journal of Biochemistry·Hideyuki HayashiToshiharu Yagi
Apr 9, 2008·Acta Crystallographica. Section D, Biological Crystallography·Erika V SorianoSteven E Ealick
Mar 15, 1993·European Journal of Biochemistry·Z Shao, E B Newman
Jan 1, 1990·Annals of the New York Academy of Sciences·E E Snell
Jan 7, 2011·Molecular Microbiology·Hein TripJuke S Lolkema
Jan 26, 2010·Essays in Biochemistry·Anthony E Pegg
Aug 31, 2000·Annual Review of Biochemistry·H Paulus
Mar 1, 2008·Journal of Molecular Biology·Anders SandbergTorleif Härd
Aug 9, 2006·Critical Reviews in Microbiology·María Fernández, Manuel Zúñiga
May 1, 2002·The Journal of Biological Chemistry·David E GrahamRobert H White
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May 10, 2011·Journal of Chemical Theory and Computation·Rong-Zhen LiaoFahmi Himo

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