PMID: 6975476Aug 1, 1981Paper

Conversion of soluble immune response suppressor to macrophage-derived suppressor factor by peroxide

Proceedings of the National Academy of Sciences of the United States of America
T M Aune, C W Pierce

Abstract

After incubation with soluble immune response suppressor (SIRS), a product of concanavalin A-activated Ly2+ T cells, macrophages release a factor that suppresses in vitro antibody responses, DNA synthetic responses to T-cell and B-cell mitogens, and division of several tumor cell lines. This factor, macrophage-derived suppressor factor (M phi-SF), is a protein with an apparent Mr of 55,000 that is inactivated by sulfhydryl compounds, certain amines, and iodide but not by other halides. In experiments reported here, conventional SIRS and SIRS produced by a cloned T-cell hybridoma were used to analyze formation of M phi-SF by SIRS-treated macrophages. Formation of M phi-SF was insensitive to inhibitors of protein and prostaglandin synthesis but was sensitive to catalase and cyanide, indicating that M phi-SF was not a newly synthesized product and that peroxide was important to its formation. As M phi-SF and SIRS have similar molecular weights and other properties, it is possible that M phi-SF is SIRS modified by peroxide. To test this possibility, SIRS was treated with H2O2 and M phi-SF activity was determined. H2O2 at 0.1-1 pM was sufficient to convert SIRS to M phi-SF; the reaction required approximately 15-20 min and was sensi...Continue Reading

References

Jan 1, 1976·Annual Review of Biochemistry·M Morrison, G R Schonbaum
Jun 1, 1972·Journal of Cellular Physiology·P CoffinoM D Scharff

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Citations

Jul 15, 1982·Klinische Wochenschrift·T SchaffnerH Cottier
Oct 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M K CathcartD W Morel
Aug 27, 1982·Journal of Immunological Methods·T M Aune, C W Pierce
Nov 28, 1985·Journal of Immunological Methods·T M Aune
Feb 1, 1993·Mechanisms of Ageing and Development·R A FranklinK W Kelley
Nov 1, 1988·Immunopharmacology·Y TomitaM K Cathcart
Jul 1, 1984·Veterinary Immunology and Immunopathology·H Bielefeldt OhmannL A Babiuk
Jan 1, 1983·International Journal of Immunopharmacology·T M Aune, C W Pierce
Jan 1, 1985·International Journal of Immunopharmacology·T M Aune
Jan 11, 1992·International Journal of Immunopharmacology·D E FrazierR G Olsen
Aug 1, 1995·Free Radical Biology & Medicine·L PackerH J Tritschler
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T M Aune, C W Pierce
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T M Aune
Apr 1, 1993·AIDS Research and Human Retroviruses·S K OhK Hartshorn
Oct 1, 1982·Cellular Immunology·R J WarringtonP J Sauder
Dec 1, 1991·Cellular Immunology·T NegoroM Watanabe
Sep 1, 1987·Scandinavian Journal of Immunology·O LindénH O Sjögren

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