Conversion of Sphingobium chlorophenolicum ATCC 39723 to a hexachlorobenzene degrader by metabolic engineering

Applied and Environmental Microbiology
Da-Zhong YanNing-Yi Zhou

Abstract

The gene cassette (camA+ camB+ camC) encoding a cytochrome P-450cam variant was integrated into the nonessential gene pcpM of the pentachlorophenol degrader Sphingobium chlorophenolicum ATCC 39723 by homologous recombination. The recombinant strain could degrade hexachlorobenzene at a rate of 0.67 nmol.mg (dry weight)-1.h-1, and intermediate pentachlorophenol was also identified.

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Citations

Oct 6, 2007·Biodegradation·Jim A Field, Reyes Sierra-Alvarez
Mar 1, 2008·Indian Journal of Microbiology·Colin ScottJohn G Oakeshott
Jul 22, 2009·Nature Chemical Biology·Shelley D Copley
May 1, 2012·Applied and Environmental Microbiology·Jun ZhangShun-Peng Li
Dec 24, 2014·World Journal of Microbiology & Biotechnology·Da-Zhong YanJun Liu
May 29, 2016·Environmental Science and Pollution Research International·Jaya Chakraborty, Surajit Das
Nov 17, 2006·Biotechnology Journal·Weihong Zhong, Jianmeng Chen
Oct 25, 2020·Applied and Environmental Microbiology·Yuan GuoNing-Yi Zhou
May 18, 2021·Frontiers in Bioengineering and Biotechnology·Jing LiWenlin An
Jun 17, 2021·Applied and Environmental Microbiology·Ling HuangHongzhi Tang

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