Convulsant bicuculline modifies CNS muscarinic receptor affinity.

BMC Neuroscience
P G Schneider, G Rodríguez de Lores Arnaiz

Abstract

Previous work from this laboratory has shown that the administration of the convulsant drug 3-mercaptopropionic acid (MP), a GAD inhibitor, modifies not only GABA synthesis but also binding of the antagonist [3H]-quinuclidinyl benzilate ([3H]-QNB) to central muscarinic receptors, an effect due to an increase in affinity without modifications in binding site number. The cholinergic system has been implicated in several experimental epilepsy models and the ability of acetylcholine to regulate neuronal excitability in the neocortex is well known. To study the potential relationship between GABAergic and cholinergic systems with seizure activity, we analyzed the muscarinic receptor after inducing seizure by bicuculline (BIC), known to antagonize the GABA-A postsynaptic receptor subtype. We analyzed binding of muscarinic antagonist [3H]-QNB to rat CNS membranes after i.p. administration of BIC at subconvulsant (1.0 mg/kg) and convulsant (7.5 mg/kg) doses. Subconvulsant BIC dose failed to develop seizures but produced binding alteration in the cerebellum and hippocampus with roughly 40% increase and 10% decrease, respectively. After convulsant BIC dose, which invariably led to generalized tonic-clonic seizures, binding increased 36% ...Continue Reading

References

Jan 1, 1991·Experimental Brain Research·G G HwaJ G Villemure
Jun 1, 1991·Annals of Neurology·W T BlumeJ P Girvin
Jan 1, 1989·British Journal of Clinical Pharmacology·B S Meldrum
Sep 1, 1973·Journal of Neurochemistry· Rodríguez de Lores ArnM Mistrorigo de Pacheco
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H I Yamamura, S H Snyder
Sep 30, 1969·Annals of the New York Academy of Sciences·H SprinceJ Magazino
May 1, 1972·Journal of Neurochemistry· Rodríguez de Lores ArnE De Robertis
Mar 1, 1995·Journal of Neurobiology·M KhrestchatiskyY Ben-Ari
Jan 1, 1994·Life Sciences·N W Pedigo
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·S E HamiltonN M Nathanson
Jan 23, 1998·Molecular and Chemical Neuropathology·P G Schneider, G Rodríguez de Lores Arnaiz
May 20, 1999·The Journal of Physiology·S FucileP Bregestovski
Mar 30, 2001·Neuroscience Letters·S Potier, C Psarropoulou
Oct 16, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rivelilson Mendes FreitasMarta Maria de F Fonteles
Jan 1, 1995·Epilepsia·B S Meldrum

❮ Previous
Next ❯

Software Mentioned

EBDA

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.