Cooling of the oral mucosa to prevent adverse effects of chemotherapeutic agents: An in vitro study

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Java WalladbegiHelen E Colley

Abstract

The cytotoxic effect of chemotherapeutic agents to the oral mucosa, as a side effect of cancer treatment, is a major problem. Cooling the oral mucosa using ice chips in conjunction with chemotherapy is known to reduce the severity of oral mucositis. However, although the use of ice chips is of clinical value, this method of cooling has inherent problems including discomfort for the patient, non-uniformity and fluctuations in cooling temperature throughout the oral cavity. Furthermore, despite being used clinically, it is not known what reduction in temperature is required to prevent oral mucositis. The aim of this study was therefore to determine in vitro if the cytotoxic effect of 5-fluorouracil (5-FU) on the oral mucosa could be reduced by lowering the temperature during chemotherapeutic treatment. Tissue-engineered oral mucosal (TEOM) models were incubated at 20, 25, 30 or 35°C for 30 minutes followed by exposure to a clinically relevant concentration of 5-FU (162 μg/mL) for 2 hours and compared with untreated models (35°C). Cell viability and inflammatory cytokine production (IL-6 and TNF-α) were measured using PrestoBlue® and ELISA, respectively. TEOM models incubated at 20°C showed an increased cell viability and had a re...Continue Reading

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Citations

Dec 15, 2019·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·M Elvira P CorreaSharon Elad
Jan 30, 2020·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Tove KamsvågGustaf Ljungman
Dec 2, 2020·International Journal of Molecular Sciences·Ayana OsakiNoriatsu Shigemura
Jul 3, 2021·Tissue Engineering. Part C, Methods·Bethany OllingtonCraig Murdoch

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