Cooling reduces cAMP-stimulated exocytosis and adiponectin secretion at a Ca2+-dependent step in 3T3-L1 adipocytes

PloS One
Mickaël F El HachmaneCharlotta S Olofsson

Abstract

We investigated the effects of temperature on white adipocyte exocytosis (measured as increase in membrane capacitance) and short-term adiponectin secretion with the aim to elucidate mechanisms important in regulation of white adipocyte stimulus-secretion coupling. Exocytosis stimulated by cAMP (included in the pipette solution together with 3 mM ATP) in the absence of Ca2+ (10 mM intracellular EGTA) was equal at all investigated temperatures (23°C, 27°C, 32°C and 37°C). However, the augmentation of exocytosis induced by an elevation of the free cytosolic [Ca2+] to ~1.5 μM (9 mM Ca2+ + 10 mM EGTA) was potent at 32°C or 37°C but less distinct at 27°C and abolished at 23°C. Adiponectin secretion stimulated by 30 min incubations with the membrane permeable cAMP analogue 8-Br-cAMP (1 mM) or a combination of 10 μM forskolin and 200 μM IBMX was unaffected by a reduction of temperature from 32°C to 23°C. At 32°C, cAMP-stimulated secretion was 2-fold amplified by inclusion of the Ca2+ ionophore ionomycin (1μM), an effect that was not observed at 23°C. We suggest that cooling affects adipocyte exocytosis/adiponectin secretion at a Ca2+-dependent step, likely involving ATP-dependent processes, important for augmentation of cAMP-stimulate...Continue Reading

References

Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Lindau, E Neher
Jan 28, 2003·Lancet·Joachim SprangerAndreas F H Pfeiffer
Mar 29, 2003·Physiological Reviews·Robert D Burgoyne, Alan Morgan
May 20, 2004·Molecular and Cellular Endocrinology·Aina S LihnBjørn Richelsen
Dec 14, 2004·Biochimica Et Biophysica Acta·Pontus Aspenström
Sep 1, 2005·The Journal of Biological Chemistry·Avirup BoseMichael P Czech
Jan 16, 2008·The Journal of General Physiology·Zhen Zhang, Meyer B Jackson
Nov 18, 2008·Metabolism: Clinical and Experimental·Regje M E BlümerPeter F Dubbelhuis
Sep 3, 2009·Molecular and Cellular Endocrinology·Sandra GalicGregory R Steinberg
May 24, 2012·Biochemical and Biophysical Research Communications·Karin Åström-OlssonLars Grip
Dec 10, 2013·Trends in Biochemical Sciences·Mikael EliasDan S Tawfik

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Citations

Jan 18, 2018·The Biochemical Journal·Mickaël F El HachmaneCharlotta S Olofsson
Oct 28, 2019·Physiological Reports·Hana InoueUtako Yokoyama
Oct 22, 2020·NPJ Breast Cancer·Natascia MarinoAnna Maria V Storniolo
Dec 3, 2020·Biochemical and Biophysical Research Communications·Saliha MusovicCharlotta S Olofsson
Aug 28, 2021·International Journal of Molecular Sciences·Marina Ruxandra OteleaAgripina Rascu

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