Cooperation between actin-binding proteins of invasive Salmonella: SipA potentiates SipC nucleation and bundling of actin

The EMBO Journal
E J McGhieVassilis Koronakis

Abstract

Pathogen-induced remodelling of the host cell actin cytoskeleton drives internalization of invasive Salmonella by non-phagocytic intestinal epithelial cells. Two Salmonella actin-binding proteins are involved in internalization: SipC is essential for the process, while SipA enhances its efficiency. Using purified SipC and SipA proteins in in vitro assays of actin dynamics and F-actin bundling, we demonstrate that SipA stimulates substantially SipC-mediated nucleation of actin polymerization. SipA additionally enhances SipC-mediated F-actin bundling, and SipC-SipA collaboration generates stable networks of F-actin bundles. The data show that bacterial SipC and SipA cooperate to direct efficient modulation of actin dynamics, independently of host cell proteins. The ability of SipA to enhance SipC-induced reorganization of the actin cytoskeleton in vivo was confirmed using semi-permeabilized cultured mammalian cells.

References

Oct 1, 1987·The Journal of Cell Biology·J A Cooper
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Feb 1, 1984·Journal of General Microbiology·S YamaguchiT Iino
Feb 25, 1998·Biochemical and Biophysical Research Communications·H Miki, T Takenawa
Mar 4, 1998·Current Opinion in Cell Biology·K R Ayscough
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·F A NorrisP W Majerus
Feb 27, 1999·Current Opinion in Microbiology·G T Nhieu, P J Sansonetti
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MacheskyT D Pollard
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·D ZhouJ E Galán
Sep 16, 1999·The EMBO Journal·R D Hayward, V Koronakis
Nov 5, 1999·Trends in Biochemical Sciences·T M Svitkina, G G Borisy

❮ Previous
Next ❯

Citations

Oct 11, 2011·Cellular and Molecular Life Sciences : CMLS·C V SrikanthBeth A McCormick
Oct 24, 2002·FEMS Microbiology Letters·H Sandovsky-LosicaE Segal
Jan 16, 2002·Current Opinion in Immunology·Steven Greenberg, Sergio Grinstein
Feb 21, 2002·Trends in Cell Biology·Richard D Hayward, Vassilis Koronakis
Jul 4, 2007·Expert Reviews in Molecular Medicine·Abigail N Layton, Edouard E Galyov
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·L A KnodlerB B Finlay
Apr 12, 2005·Nature Reviews. Microbiology·Klaus Aktories, Joseph T Barbieri
Nov 21, 2007·Nature Reviews. Microbiology·Andrea HaragaSamuel I Miller
Mar 10, 2010·The Journal of Biological Chemistry·Sebenzile K Myeni, Daoguo Zhou
Jun 15, 2006·Medical Mycology·Hana Sandovsky-LosicaEsther Segal
Apr 15, 2005·Molecular Biology of the Cell·Ming YanWilliam S Trimble
Dec 24, 2009·Infection and Immunity·Cristiano G MoreiraVanessa Sperandio
Sep 18, 2007·Journal of Bacteriology·Gen-ichiro SanoKoichi Matsuo
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Joseph T BarbieriKlaus Aktories
Apr 5, 2008·PLoS Pathogens·Robert J CainVassilis Koronakis
Jun 19, 2012·Future Microbiology·Joris van der Heijden, B Brett Finlay
Aug 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Markus C SchlumbergerWolf-Dietrich Hardt
Oct 14, 2014·International Journal of Molecular Sciences·Jun Hyuck LeeYong Ho Park
Oct 21, 2004·The Journal of Pathology·Klemens RottnerTheresia E B Stradal
Feb 29, 2008·Current Opinion in Microbiology·Olivia Steele-Mortimer
Sep 30, 2015·Pathogens and Disease·Joana N BugalhãoIrina S Franco
Aug 13, 2003·Traffic·Leigh A Knodler, Olivia Steele-Mortimer
Sep 3, 2003·Molecular Microbiology·Yoram ShotlandEduardo A Groisman
Jun 27, 2007·Cellular Microbiology·Kim Thien Ly, James E Casanova
Dec 3, 2008·Cellular Microbiology·Charlotte A Perrett, Mark A Jepson
Dec 14, 2007·Cell Motility and the Cytoskeleton·David PoppYuichiro Maéda
Jan 4, 2012·Proteomics. Clinical Applications·Sylvia SchlekerJudith Klein-Seetharaman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.