Cooperation between HNF-1alpha, Cdx2, and GATA-4 in initiating an enterocytic differentiation program in a normal human intestinal epithelial progenitor cell line.

American Journal of Physiology. Gastrointestinal and Liver Physiology
Yannick D BenoitJean-François Beaulieu

Abstract

In the intestinal epithelium, the Cdx, GATA, and HNF transcription factor families are responsible for the expression of differentiation markers such as sucrase-isomaltase. Although previous studies have shown that Cdx2 can induce differentiation in rat intestinal IEC-6 cells, no data are available concerning the direct implication of transcription factors on differentiation in human normal intestinal epithelial cell types. We investigated the role of Cdx2, GATA-4, and HNF-1alpha using the undifferentiated human intestinal epithelial crypt cell line HIEC. These transcription factors were tested on proliferation and expression of polarization and differentiation markers. Ectopic expression of Cdx2 or HNF-1alpha, alone or in combination, altered cell proliferation abilities through the regulation of cyclin D1 and p27 expression. HNF-1alpha and GATA-4 together induced morphological modifications of the cells toward polarization, resulting in the appearance of functional features such as microvilli. HNF-1alpha was also sufficient to induce the expression of cadherins and dipeptidylpeptidase, whereas in combination with Cdx2 it allowed the expression of the late differentiation marker sucrase-isomaltase. Large-scale analysis of gene...Continue Reading

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Citations

Oct 11, 2011·Digestive Diseases and Sciences·Hao ChenXiaoxin Chen
Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Keyan SalariJonathan R Pollack
Apr 3, 2012·Journal of Cell Science·Yannick D BenoitJean-François Beaulieu
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Jun 2, 2012·Autophagy·Jean-Francois GroulxJean-François Beaulieu
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Apr 12, 2016·Journal of Cellular Physiology·Alireza RoostaeeJean-François Beaulieu
May 9, 2019·Journal of Molecular Cell Biology·Katrine NørgaardAntoaneta Belcheva
Nov 19, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anders Krüger OlsenJesper Thorvald Troelsen
Apr 25, 2019·Nature Communications·Jérémie RispalFabrice Escaffit
Jul 2, 2015·Journal of Cellular Biochemistry·Alireza RoostaeeJean-François Beaulieu
Aug 23, 2020·Cells·Sepideh Fallah, Jean-François Beaulieu
Aug 4, 2010·Biochemical and Biophysical Research Communications·Yannick D BenoitJean-François Beaulieu

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