Cooperative binding of LysM domains determines the carbohydrate affinity of a bacterial endopeptidase protein

The FEBS Journal
Jaslyn E M M WongM Blaise

Abstract

Cellulose, chitin and peptidoglycan are major long-chain carbohydrates in living organisms, and constitute a substantial fraction of the biomass. Characterization of the biochemical basis of dynamic changes and degradation of these β,1-4-linked carbohydrates is therefore important for both functional studies of biological polymers and biotechnology. Here, we investigated the functional role of multiplicity of the carbohydrate-binding lysin motif (LysM) domain that is found in proteins involved in bacterial peptidoglycan synthesis and remodelling. The Bacillus subtilis peptidoglycan-hydrolysing NlpC/P60 D,L-endopeptidase, cell wall-lytic enzyme associated with cell separation, possesses four LysM domains. The contribution of each LysM domain was determined by direct carbohydrate-binding studies in aqueous solution with microscale thermophoresis. We found that bacterial LysM domains have affinity for N-acetylglucosamine (GlcNac) polymers in the lower-micromolar range. Moreover, we demonstrated that a single LysM domain is able to bind carbohydrate ligands, and that LysM domains act additively to increase the binding affinity. Our study reveals that affinity for GlcNAc polymers correlates with the chain length of the carbohydrate,...Continue Reading

References

Jan 1, 1994·European Biophysics Journal : EBJ·J S PedersenR Bauer
Oct 17, 2006·Journal of Bacteriology·Catherine EckertStéphane Mesnage
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ayako MiyaNaoto Shibuya
Dec 18, 2007·The Journal of Biological Chemistry·Takayuki OhnumaEtsuko Katoh
Apr 24, 2008·Molecular Microbiology·Girbe BuistOscar P Kuipers
Sep 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Emma J HayhurstSimon J Foster
Jan 10, 2009·Nature Protocols·Lorenza BordoliTorsten Schwede
Apr 23, 2009·Journal of Molecular Biology·Cristiano Luis Pinto OliveiraJan Skov Pedersen
Dec 3, 2009·The Journal of Biological Chemistry·Ei'ichi IizasaYukio Nagano
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Aug 21, 2010·Science·Ronnie de JongeBart P H J Thomma
Nov 13, 2010·The Plant Journal : for Cell and Molecular Biology·Takeo ShimizuNaoto Shibuya
May 28, 2011·Advances in Experimental Medicine and Biology·Pierre RougéAnnick Barre
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Roland WillmannThorsten Nürnberger
Dec 1, 2011·Nucleic Acids Research·Marco PuntaRobert D Finn
Dec 29, 2011·Nature Reviews. Microbiology·Athanasios TypasWaldemar Vollmer
May 15, 2012·Trends in Plant Science·Andrea A GustThorsten Nürnberger
Jun 2, 2012·Science·Tingting LiuJijie Chai
Jun 21, 2012·Analytical Biochemistry·Dejan M PetrovićJaap Broos
Aug 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Angelique BroghammerJens Stougaard
Dec 29, 2012·Methods : a Companion to Methods in Enzymology·Susanne A I SeidelStefan Duhr
Apr 1, 2012·Journal of Applied Crystallography·Maxim V PetoukhovDmitri I Svergun

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Citations

Oct 13, 2015·Structure·Leonardus M I KoharudinAngela M Gronenborn
Nov 13, 2014·Molecular Microbiology·Daniela ProençaCarlos São-José
Mar 1, 2015·FEMS Microbiology Reviews·Andrea Sánchez-ValletBart P H J Thomma
Jul 1, 2014·Nature Communications·Stéphane MesnageMichael P Williamson
Feb 15, 2019·Frontiers in Plant Science·Sofie Van Holle, Els J M Van Damme
Aug 1, 2020·Nature Communications·Jaslyn E M M WongKasper R Andersen
Jan 18, 2019·Nature Communications·Robert M CleverleyRichard J Lewis
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·Jaslyn E M M WongMickaël Blaise
Mar 2, 2018·Frontiers in Microbiology·Mickaël DesvauxPascale Serror
Apr 14, 2019·Scientific Reports·Paweł MitkowskiIzabela Sabała
Apr 16, 2019·Frontiers in Microbiology·Oihane IrazokiFelipe Cava
Jul 22, 2021·Cell Reports·Xin HeChristopher B Lawrence
Oct 12, 2021·Chemistry : a European Journal·Antoine RousseauSébastien Fort

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