Cooperative binding properties of restriction endonuclease EcoRII with DNA recognition sites.

The Journal of Biological Chemistry
Monika ReuterDetlev H Krüger

Abstract

EcoRII is a member of the expanding group of type IIe restriction endonucleases that share the distinguishing feature of requiring cooperativity between two recognition sites in their substrate DNA. To determine the stoichiometry of the active DNA-enzyme complex and the mode of cooperative interaction, we have investigated the dependence of EcoRII cleavage on the concentration of EcoRII dimers. Maximal restriction was observed at dimer/site ratios of 0.25 and 0. 5. The molecular weight of the DNA-enzyme complex eluted from a gel filtration column also corresponds to a dimeric enzyme structure bound to two substrate sites. We conclude that one EcoRII dimer is sufficient to interact cooperatively with two DNA recognition sites. A Lac repressor "barrier" bound between two normally reactive EcoRII sites did not inhibit restriction endonuclease activity, indicating that cooperativity between EcoRII sites is achieved by bending or looping of the intervening DNA stretch. Comparative cleavage of linear substrates with differently spaced interacting sites revealed an inverse correlation between cleavage rate and site distance. At the optimal distance of one helical turn, EcoRII cleavage is independent of the orientation of the recogniti...Continue Reading

References

Apr 1, 1992·Gene·F Wright, M J Bibb
Mar 1, 1992·Microbiological Reviews·K S Matthews
Feb 19, 1991·Biochemistry·M D TopalJ Griffith
May 11, 1988·Nucleic Acids Research·D H KrügerH O Smith
Feb 1, 1986·Journal of Bacteriology·P Sadowski
Apr 15, 1988·Science·J C Wang, G N Giaever
Feb 15, 1994·Biochemical and Biophysical Research Communications·O V PetrauskeneW Guschlbauer
Feb 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Dröge
Jun 1, 1993·Microbiological Reviews·T A Bickle, D H Krüger
Dec 1, 1995·Trends in Biochemical Sciences·K RippeJ Langowski
Dec 1, 1996·Current Opinion in Structural Biology·D E Koshland
Jan 1, 1952·Advances in Protein Chemistry·G H BEAVEN, E R HOLIDAY

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Citations

Mar 22, 2000·Journal of Biotechnology·K RuscherA Meisel
Jul 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·M FojtováR Matyásek
Mar 28, 2008·The Journal of Biological Chemistry·Shuntaro TakahashiYoshio Okahata
Oct 3, 2002·The EMBO Journal·Merlind MückeMonika Reuter
Mar 26, 2003·Nucleic Acids Research·Richard J RobertsShuang-yong Xu
Oct 25, 2003·Nucleic Acids Research·Merlind MuckeMonika Reuter
Jun 24, 2010·Nucleic Acids Research·Mindaugas ZarembaVirginijus Siksnys
Jul 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gregory J GemmenDouglas E Smith
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jurate Bitinaite, Ira Schildkraut
May 26, 2007·Journal of Molecular Biology·Eva Scheuring VanameeAneel K Aggarwal
Jan 15, 2008·Biophysical Journal·Neeraj J AgrawalPrashant K Purohit
Nov 26, 2005·The EMBO Journal·Eva Scheuring VanameeAneel K Aggarwal
Aug 21, 2007·Analytical Biochemistry·Oksana V DenisovaNicholas I Matvienko
Jun 1, 2014·Nucleic Acids Research·Alfred PingoudWolfgang Wende
Sep 12, 2006·Biophysical Journal·Gregory J GemmenDouglas E Smith
Sep 5, 2009·Nucleic Acids Research·Dmitrij GolovenkoVirginijus Siksnys
Aug 12, 1998·Journal of Molecular Biology·T J NobbsS E Halford
Dec 1, 2001·The Journal of Biological Chemistry·Abigail J BathStephen E Halford
Dec 14, 1999·The Journal of Biological Chemistry·D T BilcockS E Halford
Feb 8, 2002·The Journal of Biological Chemistry·Merlind MückeMonika Reuter
Mar 4, 2000·The Journal of Biological Chemistry·N A GormleyS E Halford
May 10, 2017·FEBS Letters·Elena M IbryashkinaMarina V Zakharova
Apr 30, 2019·Advanced Materials·Daniela S MattesFrank Breitling
Sep 12, 2007·Biochemistry·Luda S ShlyakhtenkoYuri L Lyubchenko
Jun 17, 1999·Journal of Molecular Biology·M L EmbletonS E Halford
Oct 16, 1999·Journal of Molecular Biology·V SiksnysR Huber

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