PMID: 2106914Mar 1, 1990Paper

Cooperative effect of fructose bisphosphate and glyceraldehyde-3-phosphate dehydrogenase on aldolase action

Biochimica Et Biophysica Acta
J NeuzilJ Ovádi

Abstract

The combination of binding and kinetic approaches is suggested to study (i) the mechanism of substrate-modulated dynamic enzyme associations; (ii) the specificity of enzyme interactions. The effect of complex formation between aldolase and glyceraldehyde-3-phosphate dehydrogenase (D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12) on aldolase catalysis was investigated under pseudo-first-order conditions. No change in kcat but a significant increase in KM of fructose 1,6-bisphosphate for aldolase was found when both enzymes were obtained from muscle. In contrast, kcat rather than KM changed if dehydrogenase was isolated from yeast. Next, the conversion of fructose 1-phosphate was not affected by interactions between enzyme couples isolated from muscle. The influence of fructose phosphates on the enzyme-complex formation was studied by means of covalently attached fluorescent probe. We found that the interaction ws not perturbed by the presence of fructose 1-phosphate; however, fructose 1,6-bisphosphate altered the dissociation constant of the enzyme complex. A molecular model for fructose 1,6-bisphosphate-modulated enzyme interaction has been evaluated which suggests that high levels of fructose bi...Continue Reading

References

Apr 1, 1978·European Journal of Biochemistry·J Ovádi, T Keleti
Jan 1, 1987·Annual Review of Biochemistry·P A Srere
Oct 1, 1987·Archives of Biochemistry and Biophysics·G D Reinhart, S B Hartleip
Jan 1, 1988·Current Topics in Cellular Regulation·T Keleti, J Ovádi
Nov 28, 1986·Science·D K Srivastava, S A Bernhard
Jan 1, 1981·CRC Critical Reviews in Biochemistry·C J Masters

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Citations

Dec 1, 1996·Cell Biochemistry and Function·J Ovádi, P A Srere
Sep 7, 1991·Journal of Theoretical Biology·G R Welch, P R Marmillot
May 7, 2005·Journal of Molecular Biology·Stephan SchmitzClaudia Veigel
May 25, 2016·International Journal of Biological Macromolecules·Vladimir I MuronetzElena V Schmalhausen
May 1, 1994·Trends in Biochemical Sciences·G R Welch, J S Easterby
Nov 5, 2021·Protein Science : a Publication of the Protein Society·Kathryn D FentonAudrey L Lamb

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