Abstract
Although mouse liver contains a large population of B cells, little is known about how hepatic B cells respond to bacterial lipopolysaccharide (LPS). The cytokine and IgM productions of hepatic B cells were compared with those of splenic B cells. The effect of LPS-treated hepatic B cells on IFN-gamma production from co-cultured NK1.1+ cells was also examined by irradiation and transwell experiments. Hepatic B cells stimulated with LPS produced substantial amounts of IFN-gamma and IL-12 but a small amount of IgM, while splenic B cells did not produce any of these cytokines but produced a large amount of IgM. The hepatic B cells expressed surface markers similar to those on spleen B cells but expressed more C-X-C chemokine receptor 3 than spleen B cells. Notably, depletion of B220+ cells from liver MNCs (but not from spleen MNCs) greatly decreased LPS-induced IFN-gamma production. Furthermore, LPS-treated hepatic B cells stimulated liver NK1.1+ cells to produce a remarkable amount of IFN-gamma, not only through their soluble factors but also through direct cell-cell contact. Liver B cells may play an important role in the defense against gram-negative bacterial infections by inducing IFN-gamma production from liver NK cells.
References
Aug 1, 1990·Scandinavian Journal of Immunology·J C BeckerR E Schmidt
Sep 1, 1989·The Journal of Experimental Medicine·M KobayashiG Trinchieri
Dec 1, 1989·European Journal of Immunology·X XiaY S Choi
Oct 1, 1989·The Journal of Experimental Medicine·E B SmelandT Espevik
Jan 1, 1995·Annual Review of Immunology·G Trinchieri
Aug 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A TsukaharaT Abo
Jul 11, 1998·The Journal of Biological Chemistry·Y WengJ A DeMartino
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H DobashiI Sekine
Mar 25, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G L SuS C Wang
Jul 6, 2000·The Journal of Experimental Medicine·H OgataA Tarakhovsky
Oct 6, 2000·Science·S Fagarasan, T Honjo
Oct 24, 2000·Immunological Reviews·Q VosJ J Mond
Feb 28, 2002·Blood·Yoshinori NagaiKensuke Miyake
Mar 1, 2002·Immunology and Cell Biology·Ian R Mackay
Apr 26, 2003·Blood·Norihito YazawaKunihiko Tamaki
Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yong-Han PaikDavid A Brenner
Feb 6, 2004·Nature Immunology·Stefano CasolaKlaus Rajewsky
Jul 27, 2004·Annals of Surgery·Manabu KinoshitaHoshio Hiraide
Oct 16, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mark A Exley, Margaret James Koziel
Citations
Nov 27, 2008·Journal of Gastroenterology and Hepatology·Atsushi MatsumotoShuhji Seki
Aug 10, 2011·Infection and Immunity·María P Jiménez de BagüésJulián Pardo
Mar 29, 2014·PloS One·Atsushi SatoShuhji Seki
Mar 14, 2009·FEMS Immunology and Medical Microbiology·Ashlesh K MurthyBernard P Arulanandam
Aug 14, 2009·JPEN. Journal of Parenteral and Enteral Nutrition·Jiro OmataKazuo Hase
Aug 22, 2013·Journal of Leukocyte Biology·Masami IkarashiShuhji Seki
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paula Jennings, Dorothy Yuan
Apr 27, 2013·Clinical and Experimental Immunology·H ZhangA W Thomson
Feb 8, 2019·JCI Insight·Natalia F SmirnovaOliver Eickelberg
Mar 7, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Laura Gomez-SantosNaiara Beraza