Cooperative interaction between metallosurfactants, derived from the [Ru(2,2'-bpy)3](2+) complex, and DNA

Colloids and Surfaces. B, Biointerfaces
J A LebronP Lopez-Cornejo

Abstract

With the idea of improving and advancing the design and preparation of new reagents based on cationic surfactants for gene therapy, two luminescent metallosurfactants derived from the [Ru(2,2'-bpy)3](2+) complex were synthesized. Their interaction with DNA and the effect they exert on the conformation of the polynucleotide were studied by using different techniques. The equilibrium binding constants, Kb, of the two surfactants to DNA were obtained at different molar ratios X=[surfactant]/[DNA]. The observed sigmoidal dependence of Kb on X confirms the cooperative character of the binding. After the addition of a determined surfactant concentration, the condensation of the polymer was observed. The amount of surfactant needed to produce this conformational change is lower for the double stranded surfactant than for the single chain surfactant due to a stronger hydrophobic interaction. The addition of α-cyclodextrin molecules to the metallosurfactant/DNA solutions results in polynucleotide decompaction, which confirms the importance of the hydrophobic interactions in the condensation of the polynucleotide. Results also show the importance of choosing both a proper system to study and the most seeming measuring technique to use. I...Continue Reading

References

Nov 1, 1975·Chemico-biological Interactions·C L Greenstock, G W Ruddock
Aug 19, 2006·Advances in Colloid and Interface Science·P C GriffithsR Watanesk
Jun 23, 2009·The Journal of Physical Chemistry. B·T Lopes-CostaP Lopez-Cornejo
Jul 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·E GruesoP Lopez-Cornejo
Mar 26, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·M Lopez-LopezP Lopez-Cornejo
Sep 10, 2013·Advances in Colloid and Interface Science·Artur J M Valente, Olle Söderman

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Citations

May 24, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Andrew J HallettIan A Fallis
Sep 21, 2018·Physical Chemistry Chemical Physics : PCCP·Elia GruesoR M Giráldez-Pérez
Jul 17, 2020·Chemical Communications : Chem Comm·Sandra Estalayo-AdriánThorfinnur Gunnlaugsson
May 30, 2020·Pharmaceutics·José Antonio LebrónPilar López-Cornejo

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