PMID: 7537277May 1, 1995Paper

Cooperative signaling by alpha 5 beta 1 and alpha 4 beta 1 integrins regulates metalloproteinase gene expression in fibroblasts adhering to fibronectin

The Journal of Cell Biology
P HuhtalaC H Damsky

Abstract

Rabbit synovial fibroblasts (RSF) express basal levels of the metalloproteinases (MMP) collagenase, stromelysin-1 and 92-kD gelatinase when plated on intact fibronectin (FN), but elevated levels when plated on either the central RGD-containing cell-binding region of FN (120FN) or antibody against the alpha 5 beta 1 integrin, suggesting that domains outside 120FN may suppress the induction of MMP (Werb, Z., P. M. Tremble, O. Behrendtsen, E. Crowley, and C.H. Damsky. 1989. J. Cell Biol. 109:877-889). We therefore attempted to reconstitute the basal signaling of intact FN by plating RSF on 120FN together with domains of FN outside this region. Large COOH-terminal fragments containing both the heparin-binding and HICS domains suppressed MMP when combined with 120FN. To map the active sequences, peptides from this region and larger fragments that did, or did not, include the CS-1 portion of IIICS were tested. Only CS-1 peptide, or larger fragments containing CS-1, suppressed MMP expression induced by 120FN. In contrast, peptide V from the heparin-binding region, shown previously to stimulate focal contact formation, further enhanced MMP expression by RSF when present on the substrate with 120FN. RSF expressed alpha 4 beta 1 integrin...Continue Reading

References

Apr 1, 1992·The Journal of Experimental Medicine·Y NojimaC Morimoto
Jun 13, 1992·Lancet·I R Hart, A Saini
Oct 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J E Schwarzbauer
Nov 1, 1986·The Journal of Cell Biology·P CastellaniL Zardi
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S M FrischZ Werb
May 1, 1984·The Journal of Cell Biology·J AggelerZ Werb
Aug 1, 1994·Arthritis and Rheumatism·M P VincentiC E Brinckerhoff

❮ Previous
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Citations

Mar 13, 1998·The Journal of Pathology·J L Jones, R A Walker
Mar 15, 1996·International Journal of Cancer. Journal International Du Cancer·J Heino
Nov 27, 1996·International Journal of Cancer. Journal International Du Cancer·V GouonN Kieffer
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·K TakahashiK K Tanabe
Jun 15, 1996·Journal of Cellular Biochemistry·R M Lafrenie, K M Yamada
Apr 3, 1998·Journal of Cellular Physiology·F F IsikS M Schwartz
May 8, 2001·International Journal of Cancer. Journal International Du Cancer·G J ThomasP M Speight
Nov 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Juliano
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A MasumotoM Otsuki
Jul 17, 2001·Journal of Cellular Biochemistry. Supplement·Y KapilaR Fletterick
Jun 1, 1996·Cancer Metastasis Reviews·P C Brooks
Dec 23, 2006·Cancer Metastasis Reviews·Rosandra N KaplanDavid Lyden
Sep 4, 2012·Immunologic Research·Yue GuanJohn M Hartney
Jun 5, 2004·Ageing Research Reviews·J Labat-Robert
Feb 5, 2003·Experimental Cell Research·Valeryi K LishkoTatiana P Ugarova
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Jan 19, 2000·Trends in Pharmacological Sciences·M J Humphries
Feb 25, 2000·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·G CoxK J O'Byrne
Aug 30, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·J Heino
Sep 13, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Bjorn SteffensenGustavo Zardeneta
Sep 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·A HuttenlocherC H Damsky
Sep 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·Y L KapilaP W Johnson
Dec 24, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·S E LaFlammeA M Mastrangelo
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·N Burton-WursterJ N Macleod
Aug 26, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·N Burton-WursterJ N Macleod
Jan 9, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·A WoodsJ R Couchman
Sep 17, 2002·Current Opinion in Cell Biology·Caroline H Damsky, Dusko Ilić
Oct 1, 1996·Current Opinion in Cell Biology·M J Humphries
Oct 1, 1996·Current Opinion in Cell Biology·J A Varner, D A Cheresh
Dec 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P C Brooks
Jun 27, 2002·Seminars in Cancer Biology·J Labat-Robert
Feb 5, 1999·Progress in Retinal and Eye Research·P HiscottI Grierson
Aug 1, 1997·The International Journal of Biochemistry & Cell Biology·J L Guan

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